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. 1990 Jun 1;61(5):801-9.
doi: 10.1016/0092-8674(90)90190-p.

Role of DNA replication in vaccinia virus gene expression: a naked template is required for transcription of three late trans-activator genes

J G Keck  1 , C J Baldick Jr, B Moss
Affiliations

Role of DNA replication in vaccinia virus gene expression: a naked template is required for transcription of three late trans-activator genes

J G Keck et al. Cell. .

Abstract

The DNA replication requirement for vaccinia virus late gene expression was mimicked by transfecting a late promoter-controlled reporter gene into infected cells in the presence of a DNA synthesis inhibitor. This late promoter activation block was overcome by cotransfecting either naked linear vaccinia virion DNA or three cloned viral genes encoding trans-activator polypeptides of 17, 26, and 30 kd. These newly identified trans-activator genes were independently transcribed only from replicated or transfected DNA. These data suggest a regulatory cascade in which the parental viral genome serves as a template for the RNA polymerase and early promoter-specific transcription factors that are packaged in the infectious particle; the newly replicated DNA is accessible to sequentially synthesized intermediate promoter- and late promoter-specific trans-activators.

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