Xenopus Nkx6.1 and Nkx6.2 are required for mid-hindbrain boundary development
- PMID: 23423436
- DOI: 10.1007/s00427-013-0437-9
Xenopus Nkx6.1 and Nkx6.2 are required for mid-hindbrain boundary development
Abstract
The mid-hindbrain boundary (MHB) organizer is among the best studied local organizers that patterns the midbrain and cerebellum in vertebrates and is established by a regulatory network of several transcription factors and signals, including Wnt signaling. In the present study, we found that Xenopus Nkx6.1 and Nkx6.2, two transcription factors of the Nkx homeobox family, are required for MHB formation. In Xenopus embryos, nkx6.1 and nkx6.2 are expressed in the MHB, and knockdown of either nkx6.1 or nkx6.2 by specific morpholinos disrupts the MHB expression of en2 as well as wnt1, a key regulator for vertebrate MHB formation. In the nkx6.1/nkx6.2 morphants, co-injection of wnt1 or dngsk3β mRNA, which activates Wnt signaling, rescues the expression of en2. Nkx6.1 and Nkx6.2 activate canonical Wnt signaling in reporter assays in both cultured mammalian cells and Xenopus embryos. An Nkx6.2 deletion construct, which inhibits the ability of wild type Nkx6.2 to activate Wnt signaling, also reduces the MHB expression of en2 in Xenopus embryos. These results suggest that Nkx6.1 and Nkx6.2 are involved in MHB formation in Xenopus embryos, likely by modulating wnt1 expression.
Similar articles
-
Differential and dose-dependent regulation of gene expression at the mid-hindbrain boundary by Ras-MAP kinase signaling.Brain Res. 2008 Apr 24;1206:33-43. doi: 10.1016/j.brainres.2008.01.100. Epub 2008 Mar 17. Brain Res. 2008. PMID: 18343356
-
Identification of target genes for the Xenopus Hes-related protein XHR1, a prepattern factor specifying the midbrain-hindbrain boundary.Dev Biol. 2005 Jul 1;283(1):253-67. doi: 10.1016/j.ydbio.2005.04.020. Dev Biol. 2005. PMID: 15935340
-
Otx2 can activate the isthmic organizer genetic network in the Xenopus embryo.Mech Dev. 2002 Jan;110(1-2):3-13. doi: 10.1016/s0925-4773(01)00591-3. Mech Dev. 2002. PMID: 11744364
-
Midbrain-Hindbrain Boundary Morphogenesis: At the Intersection of Wnt and Fgf Signaling.Front Neuroanat. 2017 Aug 3;11:64. doi: 10.3389/fnana.2017.00064. eCollection 2017. Front Neuroanat. 2017. PMID: 28824384 Free PMC article. Review.
-
Fgf8 signaling for development of the midbrain and hindbrain.Dev Growth Differ. 2016 Jun;58(5):437-45. doi: 10.1111/dgd.12293. Epub 2016 Jun 7. Dev Growth Differ. 2016. PMID: 27273073 Review.
Cited by
-
Prdm12 specifies V1 interneurons through cross-repressive interactions with Dbx1 and Nkx6 genes in Xenopus.Development. 2015 Oct 1;142(19):3416-28. doi: 10.1242/dev.121871. Development. 2015. PMID: 26443638 Free PMC article.
-
Xenopus Nkx6.3 is a neural plate border specifier required for neural crest development.PLoS One. 2014 Dec 22;9(12):e115165. doi: 10.1371/journal.pone.0115165. eCollection 2014. PLoS One. 2014. PMID: 25531524 Free PMC article.
-
Overexpression of NKX6.1 is closely associated with progressive features and predicts unfavorable prognosis in human primary hepatocellular carcinoma.Tumour Biol. 2015 Jun;36(6):4405-15. doi: 10.1007/s13277-015-3080-4. Epub 2015 Jan 19. Tumour Biol. 2015. PMID: 25596704
-
Novel β-carbolines against colorectal cancer cell growth via inhibition of Wnt/β-catenin signaling.Cell Death Discov. 2015 Oct 5;1:15033. doi: 10.1038/cddiscovery.2015.33. eCollection 2015. Cell Death Discov. 2015. PMID: 27551464 Free PMC article.
-
Dual roles of Akirin2 protein during Xenopus neural development.J Biol Chem. 2017 Apr 7;292(14):5676-5684. doi: 10.1074/jbc.M117.777110. Epub 2017 Feb 13. J Biol Chem. 2017. PMID: 28193841 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
