Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Mar;20(Pt 2):226-33.
doi: 10.1107/S0909049513001647. Epub 2013 Feb 7.

Synchrotron X-ray fluorescence studies of a bromine-labelled cyclic RGD peptide interacting with individual tumor cells

Erin J Sheridan  1 , Christopher J D Austin, Jade B Aitken, Stefan Vogt, Katrina A Jolliffe, Hugh H Harris, Louis M Rendina
Affiliations

Synchrotron X-ray fluorescence studies of a bromine-labelled cyclic RGD peptide interacting with individual tumor cells

Erin J Sheridan et al. J Synchrotron Radiat. 2013 Mar.

Abstract

The first example of synchrotron X-ray fluorescence imaging of cultured mammalian cells in cyclic peptide research is reported. The study reports the first quantitative analysis of the incorporation of a bromine-labelled cyclic RGD peptide and its effects on the biodistribution of endogenous elements (for example, K and Cl) within individual tumor cells.

Keywords: RGD peptide; XRF; microprobe; tumor cells.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Cell proliferation and angiogenesis requires interaction between the cell and the extracellular matrix that is mediated by integrins. Endogenous RGD peptides bind to integrins and anchor migrating and dividing cells. The desired mode of action of RGD antagonists is to block endogenous RGD peptide binding, thus preventing continued cellular proliferation and angiogenesis.
Figure 2
Figure 2
Scattered X-ray (SA) and XRF elemental distribution maps of P, S, Cl, K, Ca, Fe, Cu, Zn and Br of an B16 cell treated with 1 for 24 h. The maximal elemental area density (in µg cm−2) is given in the top corner of each map.
Figure 3
Figure 3
Scattered X-ray (SA) and XRF elemental distribution maps of P, S, Cl, K, Ca, Fe, Cu, Zn and Br of an untreated B16 cell. The maximal elemental area density (in µg cm−2) is given in the top corner of each map.
Figure 4
Figure 4
Scattered X-ray (SA) and XRF elemental distribution maps of P, S, Cl, K, Ca, Fe, Cu, Zn and Br of an A549 cell treated with 1 for 24 h. The maximal elemental area density (in µg cm−2) is given in the top corner of each map.
Figure 5
Figure 5
Scattered X-ray (SA) and XRF elemental distribution maps of P, S, Cl, K, Ca, Fe, Cu, Zn and Br of an untreated A549 cell. The maximal elemental area density (in µg cm−2) is given in the top corner of each map.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K. & Watson, J. D. (1994). Molecular Biology of the Cell. New York: Garland.
    1. Arcangeli, A. & Becchetti, A. (2006). Trends Cell Biol. 16, 631–639. - PubMed
    1. Arcangeli, A., Faravelli, L., Bianchi, L., Rosati, B., Gritti, A., Vescovi, A., Wanke, E. & Olivotto, M. (1996). Cell Adhes. Commun. 4, 369–385. - PubMed
    1. Artym, V. V. & Petty, H. R. (2002). J. Gen. Physiol. 120, 29–37. - PMC - PubMed
    1. Avraamides, C. J., Garmy-Susini, B. & Varner, J. A. (2008). Nat. Rev. Cancer, 8, 604–617. - PMC - PubMed

Publication types