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Review
. 2013 Mar 1;5(3):a013003.
doi: 10.1101/cshperspect.a013003.

Adenovirus DNA replication

Rob C Hoeben  1 , Taco G Uil
Affiliations
Review

Adenovirus DNA replication

Rob C Hoeben et al. Cold Spring Harb Perspect Biol. .

Abstract

Adenoviruses have attracted much attention as probes to study biological processes such as DNA replication, transcription, splicing, and cellular transformation. More recently these viruses have been used as gene-transfer vectors and oncolytic agents. On the other hand, adenoviruses are notorious pathogens in people with compromised immune functions. This article will briefly summarize the basic replication strategy of adenoviruses and the key proteins involved and will deal with the new developments since 2006. In addition, we will cover the development of antivirals that interfere with human adenovirus (HAdV) replication and the impact of HAdV on human disease.

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Figures

Figure 1.
Figure 1.
(A) Structure of the adenovirus type 5 origin and regions required for interaction with viral and cellular proteins. (B) Schematic representation of the initiation of DNA replication illustrating how the nucleotides 4–6 are used as a template for the formation of the pTP-CAT complex. The complex subsequently displaces to pair with nucleotides 1–3 of the template stand, after which the elongation continues.
Figure 2.
Figure 2.
Representations of AdV and ϕ29 polymerases showing the locations of conserved sequence motifs and structural domains. The domain organization shown for ϕ29 Pol was described previously by Kamtekar et al. (2004) and is based on structural data. The domain organization depicted for AdV Pol is inferred from that of ϕ29 Pol and is based on the relative location of motifs shared among (many of) the DNA polymerases. Indicated for both polymerases are the most highly conserved of these sequence motifs within the exonuclease domain (“Exo” motifs I, II, and III) and the polymerase region (“Pol” motifs I, II, and III, also known as, respectively, motifs C, A, and B) (Wong et al. 1988; Bernad et al. 1989; Delarue et al. 1990). All representations are to scale.
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