Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jun;64(6):3130-4.
doi: 10.1128/JVI.64.6.3130-3134.1990.

cis-acting elements in the U3 region of a simian immunodeficiency virus

B Renjifo  1 , N A Speck, S Winandy, N Hopkins, Y Li
Affiliations

cis-acting elements in the U3 region of a simian immunodeficiency virus

B Renjifo et al. J Virol. 1990 Jun.

Abstract

A series of 5' deletions and a point mutation in the binding site for nuclear factor kappa B were introduced into the U3 region of a molecular clone of simian immunodeficiency virus from macaques (SIVmac142). The transcriptional activity of the mutated U3 regions was analyzed by transient chloramphenicol acetyltransferase assays. Two distinct regions in U3 appeared to contain important cis-acting sequences for transcriptional activity. Mutation of the single nuclear factor kappa B site in the SIVmac142 U3 region attenuated transcription in Rat-1 fibroblasts and Jurkat T cells. A second cis-acting element was localized to sequences between -162 and -114 in U3; deletion of long terminal repeat sequences up to -114 significantly attenuated transcriptional activity in Rat-1 cells. Furthermore, sequences between -162 and -114 contributed to inducibility of transcription by 1,3-phorbol myristate acetate in Jurkat T cells. Deletion of long terminal repeat sequences to -114, in addition to mutation of the nuclear factor kappa B site, was necessary to attenuate the response to 1,3-phorbol myristate acetate completely. A negative regulatory element analogous to that identified in the U3 region from the human immunodeficiency virus was not found in the U3 region from SIVmac142.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cell. 1985 Jul;41(3):813-23 - PubMed
    1. Science. 1985 Jun 7;228(4704):1201-4 - PubMed
    1. Science. 1985 Oct 4;230(4721):71-3 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Oct;82(20):7053-7 - PubMed
    1. J Virol. 1986 Jan;57(1):379-84 - PubMed

Publication types

Substances

LinkOut - more resources