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. 2013:11013:463-82.
doi: 10.1007/978-1-62703-074-8_35.

Production of pathogen-free horticultural crops by cryotherapy of in vitro-grown shoot tips

Chaohong Feng  1 , Renrui Wang, Jingwei Li, Biao Wang, Zhenfang Yin, Zhenhua Cui, Baiquan Li, Wenlu Bi, Zhibo Zhang, Mingfu Li, Qiaochun Wang
Affiliations

Production of pathogen-free horticultural crops by cryotherapy of in vitro-grown shoot tips

Chaohong Feng et al. Methods Mol Biol. 2013.

Abstract

Horticultural crops are economically valuable for sustainable agricultural production. Plant diseases caused by Pathogens including virus, phytoplasma and bacterium have been a great threat to production of horticultural crops. The efficient use of pathogen-free plant materials has overcome the menace of plant diseases and has sustained crop production. Cryotherapy of shoot tips, a novel application of cryopreservation technique, has become a new plant biotechnology tool for plant pathogen eradication. When compared with the traditional methods, cryotherapy of shoot tips produces high frequency of pathogen-free plants, which is independent of shoot tip size and cryogenic methods. Cryotherapy of shoot tips has six major steps to produce pathogen-free plants: (1) introduction of infected plant materials into in vitro cultures; (2) excision of shoot tips; (3) cryotherapy; (4) post-culture for plant regeneration; (5) indexing of pathogens in regenerated plants after cryotherapy; and (6) establishment of pathogen-free nuclear stock plants. The key steps 2, 3, and 4 are similar to cryopreservation, and play a major role in obtaining high pathogen eradication frequency.

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