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. 2012 Nov 21:9:281.
doi: 10.1186/1743-422X-9-281.

Replication kinetics of duck enteritis virus UL16 gene in vitro

Qin He  1 , Anchun Cheng, Mingshu Wang, Jun Xiang, Dekang Zhu, Yi Zhou, Renyong Jia, Shun Chen, Zhengli Chen, Xiaoyue Chen
Affiliations

Replication kinetics of duck enteritis virus UL16 gene in vitro

Qin He et al. Virol J. .

Abstract

Background: The function and kinetics of some herpsvirus UL16 gene have been reported. But there was no any report of duck enteritis virus (DEV) UL16 gene.

Findings: The kinetics of DEV UL16 gene was examined in DEV CHv infected duck embryo fibroblasts (DEFs) by establishment of real-time quantitative reverse transcription polymerase chain reaction assay (qRT-PCR) and western-blotting. In this study, UL16 mRNA was transcript at a low level from 0-18 h post-infection (p.i), and peaked at 36 h p.i. It can't be detected in the presence of acyclovir (ACV). Besides, western-blotting analysis showed that UL16 gene expressed as an apparent 40-KDa in DEV infected cell lysate from 12 h p.i, and rose to peak level at 48 h p.i consistent with the qRT-PCR result.

Conclusions: These results provided the first evidence of the kinetics of DEV UL16 gene. DEV UL16 gene was a late gene and dependent on viral DNA synthesis.

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Figures

Figure 1
Figure 1
The qRT-PCR melt curve (a) and standard curve (b) of PMD18-T/UL16.a: the Tm of amplification fragment was 82.5°C, suggesting that the primers was specific.
Figure 2
Figure 2
The qRT-PCR melt curve (a) and standard curve (b) of PMD18-T/β-actin.a: the Tm of amplification fragment was 89.5°C, suggesting that the primers was specific.
Figure 3
Figure 3
Kinetics of DEV UL16 gene transcription.The average relative content of the DEV UL16 gene transcripts was calculated at 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 36, 48, 60 and 72 h p.i. using the 2-ΔΔCt method.
Figure 4
Figure 4
The dependence of DEV UL16 production on viral DNA synthesis. The cells were cultured in the presence(+) or absence(−) of 300 mg/ml ACV and the RNA was extracted at 36 h p.i. The RNA was inversed transcribed to cDNA. PCR was performed with cDNA.
Figure 5
Figure 5
Western-blotting of lysate from mock infected or DEV-infected DEFs with polyclonal antibodies specific to UL16 protein, showing that UL16 is expressed as a 40 kDa protein from 8 h onward following infection.
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References

    1. Fadly AM, Glisson JR, McDougald LR, Nolan L, Swayne DE. Duck Virus Enteritis. Wiley-BlackwellSaif YM, Diseases of Poultry American; 2008. pp. 384–393.
    1. King A, Lefkowita E, Adams MJ. Virus taxonomy: Ninth report of the International Committee on Taxonomy of Viruses. Elsevier. 2011. pp. 111–114.
    1. Wu Y, Cheng AC, Wang MS, Yang Q, Zhu DK, Jia RY, Chen S, Zhou Y, Wang XY, Chen XY. Complete genomic sequence of chinese virulent duck enteritis virus CHv stain. J Virol. 2012;10:5965–5965. - PMC - PubMed
    1. Xin HY, Cheng AC, Wang MS, Jia RY, Shen CJ, Chang H. Identification and Characterization of a Duck Enteritis Virus US3-Like Gene. Avian Dis. 2009;53:363–369. doi: 10.1637/8643-020409-Reg.1. - DOI - PubMed
    1. Xie W, Cheng AC, Wang MS, Chang H, Zhu DK, Luo QH. Expression and characterization of the UL31 protein from duck enteritis virus. Virol J. 2009;6:19. doi: 10.1186/1743-422X-6-19. - DOI - PMC - PubMed

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