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. 2013 Feb;59(2):207-16.
doi: 10.1016/j.ymeth.2012.11.001. Epub 2012 Nov 10.

Yeast screens for host factors in positive-strand RNA virus replication based on a library of temperature-sensitive mutants

Muhammad Shah Nawaz-ul-Rehman  1 , K Reddisiva Prasanth, Jannine Baker, Peter D Nagy
Affiliations

Yeast screens for host factors in positive-strand RNA virus replication based on a library of temperature-sensitive mutants

Muhammad Shah Nawaz-ul-Rehman et al. Methods. 2013 Feb.

Abstract

RNA viruses exploit host cells by altering cellular pathways, recruiting host factors, remodeling intracellular membranes and escaping host antiviral responses. Model hosts, such as Saccharomyces cerevisiae (yeast), are valuable to identify host factors involved in viral RNA replication. The many advantages of using yeast include the availability of various yeast mutant libraries, such as (i) single gene-deletion library; (ii) the essential gene library (yTHC); and (iii) the yeast ORF over-expression library. Here, we have used a novel temperature-sensitive (ts) mutant library of essential yeast genes to identify 118 host proteins affecting replication of Tomato bushy stunt virus, in yeast model host. Testing 787 ts mutants led to the identification of host factors, of which 72 proteins facilitated TBSV replication in yeast and 46 proteins were inhibitory. Altogether, ~85% of the identified proteins are novel host factors affecting tombusvirus replication. The ts mutant library screen also led to the identification of 17 essential genes, which have been documented before, thus confirming the importance of these genomic screens. Overall, we show the power of ts mutant library in identification of host factors for RNA virus replication.

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