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Comparative Study
. 2012 Nov 6:12:33.
doi: 10.1186/1471-213X-12-33.

Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst

Manabu Ozawa  1 , Miki Sakatani, JiQiang Yao, Savita Shanker, Fahong Yu, Rui Yamashita, Shunichi Wakabayashi, Kenta Nakai, Kyle B Dobbs, Mateus José Sudano, William G Farmerie, Peter J Hansen
Affiliations
Comparative Study

Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst

Manabu Ozawa et al. BMC Dev Biol. .

Abstract

Background: The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system.

Results: A total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM (for example, NANOG, SOX2, and STAT3) and TE (ELF5, GATA3, and KRT18) in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human.

Conclusion: Analysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast.

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Figures

Figure 1
Figure 1
Differences between inner cell mass (ICM) and trophectoderm (TE) in expression of 6 select genes as determined by quantitative PCR. Blastocysts were harvested at Day 7 and ICM and TE separated by magnetic activated cell sorting. Data represent least-squares means ± SEM of results from six biological replicates. Open bars represent ICM and filled bars TE. *=P<0.05.
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References

    1. Eckert JJ, Fleming TP. Tight junction biogenesis during early development. Biochim Biophys Acta. 2008;1778:717–728. doi: 10.1016/j.bbamem.2007.09.031. - DOI - PubMed
    1. Marikawa Y, Alarcón VB. Establishment of trophectoderm and inner cell mass lineages in the mouse embryo. Mol Reprod Dev. 2009;76:1019–1032. doi: 10.1002/mrd.21057. - DOI - PMC - PubMed
    1. Zernicka-Goetz M, Morris SA, Bruce AW. Making a firm decision: multifaceted regulation of cell fate in the early mouse embryo. Nature Rev. 2009;10:467–477. - PubMed
    1. Rossant J, Tam PPL. Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse. Development. 2009;136:701–713. doi: 10.1242/dev.017178. - DOI - PubMed
    1. Plachta N, Bollenbach T, Pease S, Fraser SE, Pantazis P. Oct4 kinetics predict cell lineage patterning in the early mammalian embryo. Nat Cell Biol. 2011;13:117–123. doi: 10.1038/ncb2154. - DOI - PubMed

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