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. 2012 Jan;7(1):10-3.

Subtyping of BK Virus in Iranian Turkish Renal Transplant Recipients by RFLP-PCR

Morteza Motazakker  1 , Morteza Bagheri, Maryam Imani
Affiliations

Subtyping of BK Virus in Iranian Turkish Renal Transplant Recipients by RFLP-PCR

Morteza Motazakker et al. Maedica (Bucur). 2012 Jan.

Abstract

Introduction: BK polyomavirus (BKV) as a member of polyomavirus family is prevalent in the human population. BKV persists in renal tissue after asymptomatic infection in childhood. The reactivation of BKV in renal transplant recipients sometimes can lead to BKV associated nephropathy. BKV isolates are classified into four serologically distinct subtypes. Present study was carried out to investigate the distribution pattern of BKV subtypes in Iranian Turkish renal transplant recipients.

Materials and methods: Urine samples from 12 kidney transplant recipients infected with BKV were analyzed by RFLP-PCR technique for classification of subtypes.

Results: Our analysis showed that all samples were infected with BKV type I. BK virus types II, III, and IV were not detected in our patients.

Conclusions: Based on the results of the present study, BKV subtype I was the most frequently detected subtype in renal transplant recipients. To our knowledge, the present study provides the first data regarding distribution of BKV subtypes in Iranian renal transplant recipients.

Keywords: BKV; recipients; renal transplant; urine.

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Conflict of interest statement

Conflict of interest: I declare that there is no conflict of interest with any others but the authors, and also the article has not been sent to any journals at the same time.

Figures

Figure 1
Figure 1. Analysis of BKV DNA types by RFLP-PCR. 327 bp products of BKV DNA from four representative samples were produced by PCR and digested with Alu I Restriction enzyme. Then PCR products that digested by Alu I consequently were digested by Xmn I. Marker: 50 bp; lane1: negative control, lane2: un-cut PCR product; lanes 3, 4, 5: cut PCR products (remind un-cut after digestion with Xmn I); and lanes 6, 7: cut PCR products with Alu I producing two fragments of 186 and 141 bp.
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