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. 2012 Nov;22(11):1609-12.
doi: 10.1038/cr.2012.141. Epub 2012 Oct 9.

A novel class of tRNA-derived small RNAs extremely enriched in mature mouse sperm

A novel class of tRNA-derived small RNAs extremely enriched in mature mouse sperm

Hongying Peng et al. Cell Res. 2012 Nov.
No abstract available

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Figures

Figure 1
Figure 1
Identification and characterization of tRNA-derived small RNAs in mature mouse sperm. (A) Length distributions of small RNAs in uterus, testis and mature sperm. (B) Alignments and statistics of small RNA family-1 and -2 in mature sperm, testis and uterus; the top three enriched RNAs for each family were highlighted. (C) Small RNA family-1 and -2 comprised the majority of 30-34 nt small RNA population. (D) Chromosome locations of small RNA family-1 and -2. (E, F) Illustrations showing that mse-tsRNA family-1 (E) and mse-tsRNA family-2 (F) are derived from 5′ halves of tRNAGlu and tRNAGly respectively; top three cleavage sites for each tRNA were marked by arrow heads. (G) Catalogue of small RNA populations in mature mouse sperm. (H) RT-PCR analyses of mse-tsRNA family-1, -2 and miR-34c in purified spermatogenic cells and mature sperm, followed by product sequencing. The variable nucleotides were marked by shade. Samples for RT-PCR were equally loaded by calibration of RNA concentration. Similar results were obtained in 3 independent experiments. (I, J) Percentages of mse-tsRNA family-1 (I) and -2 (J) reads from deep-sequencing datasets of purified spermatogenic cells and mature sperm. (K) Representative photos of intact sperm and purified sperm heads. (L) RT-PCR analyses of mse-tsRNA family-1, -2 and miR-34c in intact sperm and purified sperm heads. (M) Evolutionary tree showing that the tRNA precursors of mse-tsRNA family-1 and -2 are highly conserved in vertebrate species. (N) RT-PCR analyses of mse-tsRNA family-1 and -2 in mature sperm from mouse, rat and human.

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