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. 2012 Sep;8(9):774-83.
doi: 10.1038/nchembio.1028. Epub 2012 Jul 22.

Transient GPI-anchored protein homodimers are units for raft organization and function

Kenichi G N Suzuki  1 , Rinshi S Kasai, Koichiro M Hirosawa, Yuri L Nemoto, Munenori Ishibashi, Yoshihiro Miwa, Takahiro K Fujiwara, Akihiro Kusumi
Affiliations

Transient GPI-anchored protein homodimers are units for raft organization and function

Kenichi G N Suzuki et al. Nat Chem Biol. 2012 Sep.

Abstract

Advanced single-molecule fluorescent imaging was applied to study the dynamic organization of raft-associated glycosylphosphatidylinositol-anchored proteins (GPI-APs) in the plasma membrane and their stimulation-induced changes. In resting cells, virtually all of the GPI-APs are mobile and continually form transient (~200 ms) homodimers (termed homodimer rafts) through ectodomain protein interactions, stabilized by the presence of the GPI-anchoring chain and cholesterol. Heterodimers do not form, suggesting a fundamental role for the specific ectodomain protein interaction. Under higher physiological expression conditions , homodimers coalesce to form hetero- and homo-GPI-AP oligomer rafts through raft-based lipid interactions. When CD59 was ligated, it formed stable oligomer rafts containing up to four CD59 molecules, which triggered intracellular Ca(2+) responses that were dependent on GPI anchorage and cholesterol, suggesting a key part played by transient homodimer rafts. Transient homodimer rafts are most likely one of the basic units for the organization and function of raft domains containing GPI-APs.

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