Methods in 2012, part 2

Editorial

. 2012 Jul;53(1):11.

doi: 10.2144/000113882.

Free article

Editorial

Nathan S Blow. Biotechniques. 2012 Jul.

Free article

. 2012 Jul;53(1):11.

doi: 10.2144/000113882.

No abstract available

Comment on

Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase.
Geiser M, Cèbe R, Drewello D, Schmitz R. Geiser M, et al. Biotechniques. 2001 Jul;31(1):88-90, 92. doi: 10.2144/01311st05. Biotechniques. 2001. PMID: 11464525
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.
Bryksin AV, Matsumura I. Bryksin AV, et al. Biotechniques. 2010 Jun;48(6):463-5. doi: 10.2144/000113418. Biotechniques. 2010. PMID: 20569222 Free PMC article.

Site-directed mutagenesis of large (13-kb) plasmids in a single-PCR procedure.
Wang J, Wilkinson MF. Wang J, et al. Biotechniques. 2000 Nov;29(5):976-8. doi: 10.2144/00295bm09. Biotechniques. 2000. PMID: 11084857 No abstract available.
Cloning PCR products using T-vectors.
Trower MK, Elgar GS. Trower MK, et al. Methods Mol Biol. 1996;58:313-24. doi: 10.1385/0-89603-402-X:313. Methods Mol Biol. 1996. PMID: 8713878 No abstract available.
A high-yield method for site-directed mutagenesis using polymerase chain reaction and three primers.
Steinberg RA, Gorman KB. Steinberg RA, et al. Anal Biochem. 1994 May 15;219(1):155-7. doi: 10.1006/abio.1994.1246. Anal Biochem. 1994. PMID: 8059944 No abstract available.
A simple method to introduce internal deletions or mutations into any position of a target DNA sequence.
Tomic-Canic M, Bernerd F, Blumenberg M. Tomic-Canic M, et al. Methods Mol Biol. 1996;57:249-57. doi: 10.1385/0-89603-332-5:249. Methods Mol Biol. 1996. PMID: 8850011 Review. No abstract available.
Multiple site-directed mutagenesis.
Bhat KS. Bhat KS. Methods Mol Biol. 1996;57:269-77. doi: 10.1385/0-89603-332-5:269. Methods Mol Biol. 1996. PMID: 8850013 Review. No abstract available.