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. 2012 Jun 24:12:76.
doi: 10.1186/1471-230X-12-76.

Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis

Nina Aagaard Poulsen  1 , Vibeke Andersen, Jens Christian Møller, Hanne Søndergaard Møller, Flemming Jessen, Stig Purup, Lotte Bach Larsen
Affiliations

Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis

Nina Aagaard Poulsen et al. BMC Gastroenterol. .

Abstract

Background: Accurate diagnostic and monitoring tools for ulcerative colitis (UC) are missing. Our aim was to describe the proteomic profile of UC and search for markers associated with disease exacerbation. Therefore, we aimed to characterize specific proteins associated with inflamed colon mucosa from patients with acute UC using mass spectrometry-based proteomic analysis.

Methods: Biopsies were sampled from rectum, sigmoid colon and left colonic flexure from twenty patients with active proctosigmoiditis and from four healthy controls for proteomics and histology. Proteomic profiles of whole colonic biopsies were characterized using 2D-gel electrophoresis, and peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for identification of differently expressed protein spots.

Results: A total of 597 spots were annotated by image analysis and 222 of these had a statistically different protein level between inflamed and non-inflamed tissue in the patient group. Principal component analysis clearly grouped non-inflamed samples separately from the inflamed samples indicating that the proteomic signature of colon mucosa with acute UC is strong. Totally, 43 individual protein spots were identified, including proteins involved in energy metabolism (triosephosphate isomerase, glycerol-3-phosphate-dehydrogenase, alpha enolase and L-lactate dehydrogenase B-chain) and in oxidative stress (superoxide dismutase, thioredoxins and selenium binding protein).

Conclusions: A distinct proteomic profile of inflamed tissue in UC patients was found. Specific proteins involved in energy metabolism and oxidative stress were identified as potential candidate markers for UC.

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Figures

Figure 1
Figure 1
Principal component analysis of the proteomic profiles of healthy control persons. Results are based on 2-DGE spot volumes. Replicate biopsies were analysed from rectum (RE), sigmoid colon (SI) and left colonic flexure (LF). Control person 1 (+), control person 2 (■), control person 3 (♦) and control person 4 (▴).
Figure 2
Figure 2
Principal component analysis of the proteomic profiles of UC patients. The results are based on results from 2-DGE spot volumes. Biopsies were from rectum (RE) and left colonic flexure (LF). LF (▴) and RE (■).
Figure 3
Figure 3
Different protein level in rectum and left colonic flexure from control persons and UC patients. (A) Glycerol-3-phosphate-dehydrogenase (spot 1) and (B) Glycerol-3-phosphate-dehydrogenase (spot 4). Representative 2-DGE images from the four groups and normalised protein expression of the same groups presented as mean ± standard error. Control persons (REC and LFC) and UC patients (REP and LFP).
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