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. 2012 May 1;68(Pt 5):535-9.
doi: 10.1107/S1744309112009347. Epub 2012 Apr 20.

Initial crystallographic studies of a small heat-shock protein from Xylella fastidiosa

Susely F S Tada  1 , Antonio Marcos Saraiva, Gabriela S Lorite, Luciana K Rosselli-Murai, Alexandre César Pelloso, Marcelo Leite dos Santos, Daniela B B Trivella, Mônica A Cotta, Anete Pereira de Souza, Ricardo Aparicio
Affiliations

Initial crystallographic studies of a small heat-shock protein from Xylella fastidiosa

Susely F S Tada et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

The ORF XF2234 in the Xylella fastidiosa genome was identified as encoding a small heat-shock protein of 17.9 kDa (HSP17.9). HSP17.9 was found as one of the proteins that are induced during X. fastidiosa proliferation and infection in citrus culture. Recombinant HSP17.9 was crystallized and surface atomic force microscopy experiments were conducted with the aim of better characterizing the HSP17.9 crystals. X-ray diffraction data were collected at 2.7 Å resolution. The crystal belonged to space group P4(3)22, with unit-cell parameters a = 68.90, b = 68.90, c = 72.51 Å, and is the first small heat-shock protein to crystallize in this space group.

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Figures

Figure 1
Figure 1
A typical crystal of HSP17.9, with maximum dimension 100 µm.
Figure 2
Figure 2
AFM topographies of an HSP17.9 crystal in the solution in which growth took place. (a) Image of the crystal surface, showing terraces, step edges and a depression (indicated by an arrow). (b, c) A closer look at the anisotropic structures found at the depression site. (d) Anisotropic structures located at the top of the crystal.
Figure 3
Figure 3
A diffraction pattern from the crystal used in data collection. A higher resolution region of the image is shown with increased contrast. Rings are drawn at 2.4, 3.2, 4.8 and 9.7 Å resolution.
See this image and copyright information in PMC

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References

    1. Azzoni, A. R., Tada, S. F. S., Rosselli, L. K., Paula, D. P., Catani, C. F., Sabino, A. A., Barbosa, J. A. R. G., Guimarães, B. G., Eberlin, M. N., Medrano, F. J. & de Souza, A. P. (2004). Protein Expr. Purif. 33, 297–303. - PubMed
    1. Bagnéris, C., Bateman, O. A., Naylor, C. E., Cronin, N., Boelens, W. C., Keep, N. H. & Slingsby, C. (2009). J. Mol. Biol. 392, 1242–1252. - PubMed
    1. Caylor, C. L., Dobrianov, I., Lemay, S. G., Kimmer, C., Kriminski, S., Finkelstein, K. D., Zipfel, W., Webb, W. W., Thomas, B. R., Chernov, A. A. & Thorne, R. E. (1999). Proteins, 36, 270–281. - PubMed
    1. De Yoreo, J. J. & Dove, P. M. (2004). Science, 306, 1301–1302. - PubMed
    1. Durbin, S. D. & Carlson, W. E. (1992). J. Cryst. Growth, 122, 71–79.

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