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. 2009 Oct;1(3):20-8.

Phage display on the base of filamentous bacteriophages: application for recombinant antibodies selection

N V Tikunova  1 , V V Morozova
Affiliations

Phage display on the base of filamentous bacteriophages: application for recombinant antibodies selection

N V Tikunova et al. Acta Naturae. 2009 Oct.

Abstract

The display of peptides and proteins on the surface of filamentous bacteriophage is a powerful methodology for selection of peptides and protein domains, including antibodies. An advantage of this methodology is the direct physical link between the phenotype and the genotype, as an analyzed polypeptide and its encoding DNA fragment exist in one phage particle. Development of phage display antibody libraries provides repertoires of phage particles exposing antibody fragments of great diversity. The biopanning procedure facilitates selection of antibodies with high affinity and specificity for almost any target. This review is an introduction to phage display methodology. It presents recombinant antibodies display in more details:, construction of phage libraries of antibody fragments and different strategies for the biopanning procedure.

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Figures

Fig. 1
Fig. 1
A schematic representation of a filamentous phage: (a) wild-type filamentous phage; (b) filamentous phage-based phage antibody
Fig. 2
Fig. 2
Types of vector systems for peptide and protein display based on filamentous phages. Lilac and red represent the exogenous inserts into the genes encoding the pIII and PVIII proteins, respectively
Fig. 3
Fig. 3
Natural immunoglobulin G molecule and the antigen-binding fragments of the immunoglobulin
Fig. 4
Fig. 4
A schematic representation of the construction of an antibody fragment phage library
Fig. 5
Fig. 5
A schematic representation of the biopanning procedure
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