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Review
. 2012;13(4):5254-5277.
doi: 10.3390/ijms13045254. Epub 2012 Apr 24.

Phage displayed peptides/antibodies recognizing growth factors and their tyrosine kinase receptors as tools for anti-cancer therapeutics

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Review

Phage displayed peptides/antibodies recognizing growth factors and their tyrosine kinase receptors as tools for anti-cancer therapeutics

Roberto Ronca et al. Int J Mol Sci. 2012.

Abstract

The basic idea of displaying peptides on a phage, introduced by George P. Smith in 1985, was greatly developed and improved by McCafferty and colleagues at the MRC Laboratory of Molecular Biology and, later, by Barbas and colleagues at the Scripps Research Institute. Their approach was dedicated to building a system for the production of antibodies, similar to a naïve B cell repertoire, in order to by-pass the standard hybridoma technology that requires animal immunization. Both groups merged the phage display technology with an antibody library to obtain a huge number of phage variants, each of them carrying a specific antibody ready to bind its target molecule, allowing, later on, rare phage (one in a million) to be isolated by affinity chromatography. Here, we will briefly review the basis of the technology and the therapeutic application of phage-derived bioactive molecules when addressed against key players in tumor development and progression: growth factors and their tyrosine kinase receptors.

Keywords: anticancer therapy; growth factors; humanized antibody; phage display; tyrosine kinase receptors.

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Figures

Figure 1
Figure 1
Type of antibody formats, as mentioned in the text, with the relative molecular weight. Adapted from Holliger and Hudson [16].
Figure 2
Figure 2
Schematic example of some tyrosine kinase growth factor receptors. Adapted from Lemmon and Schlessinger [17].

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