Two steps forward--one step back: advances in affinity purification mass spectrometry of macromolecular complexes
- PMID: 22592981
- DOI: 10.1002/pmic.201100509
Two steps forward--one step back: advances in affinity purification mass spectrometry of macromolecular complexes
Abstract
Cellular functions are defined by the dynamic interactions of proteins within macromolecular networks. Deciphering these complex interplays is the key to getting a comprehensive picture of cellular behavior and to understanding biological systems, from a simple bacterial cell to highly regulated neuronal cells or cancerous tissue. In the last decade, affinity purification (AP) coupled to mass spectrometry has emerged as a powerful tool to comprehensively study interaction networks and their macromolecular assemblies. This review discusses recent advances in AP approaches, from cell lysis to the importance of sample preparation and the choice of AP matrix as well as the development of different epitope tags and strategies to study dynamic interactions, with an emphasis on RNA-protein interaction networks.
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Similar articles
-
Affinity-purification coupled to mass spectrometry: basic principles and strategies.Proteomics. 2012 May;12(10):1576-90. doi: 10.1002/pmic.201100523. Proteomics. 2012. PMID: 22611051 Review.
-
Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae.Methods Mol Biol. 2016;1361:265-87. doi: 10.1007/978-1-4939-3079-1_15. Methods Mol Biol. 2016. PMID: 26483027
-
Resolving protein interactions and complexes by affinity purification followed by label-based quantitative mass spectrometry.Proteomics. 2012 May;12(10):1623-38. doi: 10.1002/pmic.201100438. Proteomics. 2012. PMID: 22610586 Review.
-
Unraveling the dynamics of protein interactions with quantitative mass spectrometry.Crit Rev Biochem Mol Biol. 2011 Jun;46(3):216-28. doi: 10.3109/10409238.2011.567244. Epub 2011 Mar 26. Crit Rev Biochem Mol Biol. 2011. PMID: 21438726 Review.
-
Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae.Methods Mol Biol. 2022;2477:195-223. doi: 10.1007/978-1-0716-2257-5_12. Methods Mol Biol. 2022. PMID: 35524119
Cited by
-
Protein complexes and neighborhoods driving autophagy.Autophagy. 2021 Oct;17(10):2689-2705. doi: 10.1080/15548627.2020.1847461. Epub 2020 Nov 13. Autophagy. 2021. PMID: 33183148 Free PMC article. Review.
-
Mapping protein interactions of sodium channel NaV1.7 using epitope-tagged gene-targeted mice.EMBO J. 2018 Feb 1;37(3):427-445. doi: 10.15252/embj.201796692. Epub 2018 Jan 15. EMBO J. 2018. PMID: 29335280 Free PMC article.
-
Chromatography-Free Purification Strategies for Large Biological Macromolecular Complexes Involving Fractionated PEG Precipitation and Density Gradients.Life (Basel). 2021 Nov 24;11(12):1289. doi: 10.3390/life11121289. Life (Basel). 2021. PMID: 34947821 Free PMC article. Review.
-
Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.Mol Cell Biol. 2013 Dec;33(23):4718-31. doi: 10.1128/MCB.00887-13. Epub 2013 Sep 30. Mol Cell Biol. 2013. PMID: 24081329 Free PMC article.
-
Characterization of L1-Ribonucleoprotein Particles.Methods Mol Biol. 2016;1400:311-38. doi: 10.1007/978-1-4939-3372-3_20. Methods Mol Biol. 2016. PMID: 26895062 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
