Transferrin receptor internalization sequence YXRF implicates a tight turn as the structural recognition motif for endocytosis
- PMID: 2257624
- DOI: 10.1016/0092-8674(90)90509-d
Transferrin receptor internalization sequence YXRF implicates a tight turn as the structural recognition motif for endocytosis
Abstract
Using detailed functional studies on 24 human transferrin receptor mutants, we identified YXRF as the internalization sequence. Provided that at least 7 residues separate this tetrapeptide from the transmembrane region, changing the tetrapeptide position within the TR cytoplasmic domain does not reduce internalization activity. Thus, any conformational determinant for internalization must be localized to the YXRF sequence. Twenty-eight tetrapeptide analogs of YXRF, found by an unbiased search of all known three-dimensional protein structures, significantly favored tight turns similar to a type I turn. Of the ten tetrapeptides most closely related to YXRF, eight were surface exposed and had tight-turn conformations, as were four of five tetrapeptides with sequences related to the low density lipoprotein receptor internalization motif, NPXY. The internalization sequences of both receptors contain aromatic residues with intervening hydrogen-bonding residues. Thus, two distinct internalization sequences favor a common structural chemistry and implicate an exposed tight turn as the recognition motif for high efficiency endocytosis.
Similar articles
-
Structural requirements for high efficiency endocytosis of the human transferrin receptor.J Inorg Biochem. 1992 Aug 15-Sep;47(3-4):209-17. doi: 10.1016/0162-0134(92)84066-v. J Inorg Biochem. 1992. PMID: 1431881
-
Transplanted LDL and mannose-6-phosphate receptor internalization signals promote high-efficiency endocytosis of the transferrin receptor.EMBO J. 1991 Nov;10(11):3247-53. doi: 10.1002/j.1460-2075.1991.tb04888.x. EMBO J. 1991. PMID: 1655415 Free PMC article.
-
Endocytosis and signals for internalization.Curr Opin Cell Biol. 1991 Aug;3(4):634-41. doi: 10.1016/0955-0674(91)90034-v. Curr Opin Cell Biol. 1991. PMID: 1663371 Review.
-
Mutational analysis of the cytoplasmic tail of the human transferrin receptor. Identification of a sub-domain that is required for rapid endocytosis.J Biol Chem. 1991 Oct 5;266(28):19006-12. J Biol Chem. 1991. PMID: 1918016
-
[A turning point in the knowledge of the structure-function-activity relations of elastin].J Soc Biol. 2001;195(2):181-93. J Soc Biol. 2001. PMID: 11727705 Review. French.
Cited by
-
Cytoplasmic domain of P-selectin (CD62) contains the signal for sorting into the regulated secretory pathway.Mol Biol Cell. 1992 Mar;3(3):309-21. doi: 10.1091/mbc.3.3.309. Mol Biol Cell. 1992. PMID: 1378326 Free PMC article.
-
The internalization signal in the cytoplasmic tail of lysosomal acid phosphatase consists of the hexapeptide PGYRHV.EMBO J. 1992 Dec;11(12):4391-9. doi: 10.1002/j.1460-2075.1992.tb05539.x. EMBO J. 1992. PMID: 1425575 Free PMC article.
-
WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop.Cell Rep. 2019 Jan 2;26(1):79-93.e8. doi: 10.1016/j.celrep.2018.12.023. Cell Rep. 2019. PMID: 30605688 Free PMC article.
-
beta1 integrins regulate keratinocyte adhesion and differentiation by distinct mechanisms.Mol Biol Cell. 2000 Feb;11(2):453-66. doi: 10.1091/mbc.11.2.453. Mol Biol Cell. 2000. PMID: 10679006 Free PMC article.
-
Structural and functional characterization of cargo-binding sites on the μ4-subunit of adaptor protein complex 4.PLoS One. 2014 Feb 3;9(2):e88147. doi: 10.1371/journal.pone.0088147. eCollection 2014. PLoS One. 2014. PMID: 24498434 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
