Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011;4(1):37-9.
doi: 10.3980/j.issn.2222-3959.2011.01.08. Epub 2011 Feb 18.

Comparison of FGFR1 expression on lens epithelial cells between adults and fetuses

Affiliations

Comparison of FGFR1 expression on lens epithelial cells between adults and fetuses

Yu-Fu Liu et al. Int J Ophthalmol. 2011.

Abstract

Aim: To study the differences of fibroblast growth factor receptor 1 (FGFR1) gene on human lens epithelial cells (HLECs) of adults and fetuses.

Methods: Indirect in situ RT-PCR was adopted for detection of FGFR1 gene. The cDNA of the mRNA in the paraffin sections of fetus and adult HLEC was synthesized by reverse transcription reaction. After PCR amplification, in situ hybridization test was performed with synthesized oligonucleotide probe and relative quantification was carried out using image analysis.

Results: HLECs of adults and fetuses expressed FGFR1 gene, the expression level was higher in fetuses than in adults. The difference between them had significance (P<0.05).

Conclusions: FGFR1 exist in HLEC and the expression is age-related, which could be one of causes of the high occurrence of post operational after-cataract in children.

Keywords: after-cataract; fibroblast growth factor receptor 1; human lens epithelial cells; indirect in situ RT-PCR.

PubMed Disclaimer

Figures

Figure 1
Figure 1. Five months of fetal
Strong expression of FGFR1 gene in HLEC (Rt-PCR×400)
Figure 2
Figure 2. Twenty-eight years of adult
Expression of FGFR1 gene in HLEC (Rt-PCR×400)

Similar articles

References

    1. Hosal BM, Biglan AW. Risk factors for secondary membrane formation after removal of pediatric cataract. J Cataract Refract Surg. 2002;28(2):302–309. - PubMed
    1. Yadav UC, Ighani-Hosseinabad F, van Kuijk FJ, Srivastava SK, Ramana KV. Prevention of posterior capsular opacification through aldose reductase inhibition. Invest Ophthalmol Vis Sci. 2009;50(2):752–759. - PMC - PubMed
    1. Robinson ML. An essential role for FGF receptor signaling in lens development. Semin Cell Dev Biol. 2006;17(6):726–740. - PMC - PubMed
    1. Liu Y, Liu H. Detection of basic fibroblast growth factor receptor protein on human lens epithelial cells. Int J Ophthalmol (Guoji Yanke Zazhi) 2008;8(8):1511–1513.
    1. Duchesne L, Tissot B, Rudd TR, Dell A, Fernig DG. N-glycosylation of fibroblast growth factor receptor 1 regulates ligand and heparan sulfate co-receptor binding. J Biol Chem. 2006;281(37):27178–27189. - PubMed

LinkOut - more resources