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. 2012:2012:960329.
doi: 10.1155/2012/960329. Epub 2012 Feb 13.

Molecular typing of dengue virus circulating in kolkata, India in 2010

Arindam Sarkar  1 , Debjani Taraphdar, Shyamalendu Chatterjee
Affiliations

Molecular typing of dengue virus circulating in kolkata, India in 2010

Arindam Sarkar et al. J Trop Med. 2012.

Abstract

Dengue is one of the major public health threats in Kolkata. Every year, blood samples with dengue-like illness are referred to us from different medical colleges and hospitals in Kolkata for the detection of dengue infection in them. In 2010, a total of 378 samples were referred to us for that purpose. All the samples were tested for the detection of IgM antibodies by ELISA method, followed by RT-PCR test for the detection of serotypes. Only 173 samples were ELISA positive. Out of 378 samples, 108 were RT-PCR positive. Out of 108 samples, 74 samples had monotypic infection with different serotypes of DENV and 33 samples had dual infections with DENV-2 and DENV-3. Only one sample had the infection with DENV-1, DENV-2, and DENV-3. DHF was found mainly among the patients, infected with multiple dengue serotypes. Only 3 dengue monotypic infected patients had suffered from DHF.

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Figures

Figure 1
Figure 1
Age and sex wise distribution of dengue IgM-positive cases in 2010.
Figure 2
Figure 2
Monthly distributions of IgM-positive dengue cases in 2010.
Figure 3
Figure 3
Results of dengue-specific RT-PCR followed by second-round nested PCR of RNA samples, showing band in 1.5% agarose gel electrophoresis stained with ethidium bromide. Lane L: 100 bp DNA ladder, Lane S1–S8 and S10: negative sample, Lane S9, S14 and S20: sample positive for DEN-2 (119 bp), Lane S11–S13 and S16–S19: sample with dual infection of DEN-2 (119 bp) and DEN-3 (290 bp), Lane S15: sample with concurrent infection of DEN-1 (482 bp), DEN-2 (119 bp), and DEN-3 (290 bp), and Lane C: negative control.
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