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. 2012:2012:743075.
doi: 10.1155/2012/743075. Epub 2012 Feb 12.

Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Hoyoung Lee  1 , Jun Kyoung Lee, Hyekyung Ha, Mee-Young Lee, Chang-Seob Seo, Hyeun Kyoo Shin
Affiliations

Angelicae Dahuricae Radix Inhibits Dust Mite Extract-Induced Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

Hoyoung Lee et al. Evid Based Complement Alternat Med. 2012.

Abstract

We examined whether Angelicae Dahuricae Radix (AR) suppresses the development of atopic dermatitis (AD)-like skin lesions induced by Dermatophagoides farinae in NC/Nga mice. To investigate the effect of AR, we measured the AD severity score, measured plasma levels of IgE and histamine, and performed histological analysis in NC/Nga mice. We also confirmed the anti-inflammatory effects of AR by measuring TARC/CCL17 production from LPS-treated RAW 264.7 cells and mRNA levels of TARC and MDC/CCL22 in TNF-α/IFN-γ-treated HaCaT cells. 10 mg/day of AR extract was applied for 4 weeks to NC/Nga mice. Both the AR extract and 0.1% tacrolimus suppressed the development of AD-like skin lesions and reduced dermatitis scores of the back and ear skin. AR extracts caused an inhibition of histological changes induced by repeated application of D. farinae and a reduction of IgE and histamine levels in plasma (P < 0.05). Furthermore, NO production in LPS-treated RAW 264.7 cells was diminished in a dose-dependent manner, and hTARC production and TARC and MDC mRNA levels in TNF-α/IFN-γ-treated HaCaT cells were diminished by AR. The inhibitory effect of AR on NO, TARC and MDC production may be associated with the suppression of AD-like skin lesions in D. farinae-induced NC/Nga mice.

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Figures

Figure 1
Figure 1
Severity scores and histological changes of treated backs and ears in NC/Nga mice. (a) Representative features and histological changes following consecutive administration of Angelicae Dahuricae Radix (AR) or tacrolimus to Dermatophagoides farinae-induced AD-like lesions on the back and ears. The images show the back and ears 4 weeks after sensitization. (b) Effect of consecutive administration of AR or tacrolimus on dermatitis score of D. farinae-induced AD-like lesions on the back and ears. AR or tacrolimus were administrated once daily for 4 weeks. (c) Histological features of AD-like lesions treated with AR. The dermatitis scores were evaluated by the procedures described in Materials and Methods (mean ± SEM (n = 5), *: P < 0.05, compared with D. farinae-induced group).
Figure 2
Figure 2
Effect of Angelicae Dahuricae Radix (AR) on plasma levels of histamine and IgE in Dermatophagoides farinae-induced AD-like lesions in NC/Nga mice. The groups were untreated (cont), D. farinae plus vehicle-treated (D. farinae), D. farinae plus tacrolimus-treated (tacrolimus), and D. farinae plus AR-treated (AR) NC/Nga mice. The plasma concentrations of histamine (a) and IgE (b) were measured by ELISA (mean ± SEM, n = 5), #: P < 0.05, ##: P < 0.01 compared with control group, *: P < 0.05, **: P < 0.01 compared with D. farinae-induced group.
Figure 3
Figure 3
Inhibitory effects on NO and TARC by Angelicae Dahuricae Radix (AR). The concentrations of NO and TARC in the supernatant were determined using NO and TARC ELISA assays. (a) RAW 264.7 cells were treated with LPS (1 μg/mL) for 18 hr. AR inhibited NO production in a concentration-dependent manner (mean ± SEM (n = 5), #: P < 0.05, ##: P < 0.01 compared with control group, *: P < 0.05, **: P < 0.01 compared with the LPS-treated group). (b) HaCaT cells were treated with TNF-α plus IFN-γ (TI) (10 ng/mL each) for 24 h. TARC levels were significantly suppressed by AR (mean ± SEM, n = 5), #: P < 0.05, ##: P < 0.01 compared with control group, *: P < 0.05, **: P < 0.01 compared with TI treatment. (c) The MDC and TARC mRNA expression levels were determined by RT-PCR analysis, and AR suppressed the mRNA expression of MDC and TARC. The cells were treated with silymarin (10, 20, or 50 μg/mL), forskolin (5, 10, or 30 μg/mL), or AR (60, 150, or 300 μg/mL).
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