Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Jun;137(6):763-76.
doi: 10.1007/s00418-012-0937-0. Epub 2012 Feb 28.

Cell cycle-dependent subcellular distribution of ClC-3 in HeLa cells

Jianwen Mao  1 , Xiaobo Li, Weiqiang Chen, Bin Xu, Haifeng Zhang, Hongzhi Li, Liwei Wang, Xiaobao Jin, Jiayong Zhu, Guixian Lin, Weizhang Wang, Lixin Chen
Affiliations

Cell cycle-dependent subcellular distribution of ClC-3 in HeLa cells

Jianwen Mao et al. Histochem Cell Biol. 2012 Jun.

Abstract

Chloride channel-3 (ClC-3) is suggested to be a component and/or a regulator of the volume-activated Cl(-) channel in the plasma membrane. However, ClC-3 is predominantly located inside cells and the role of intracellular ClC-3 in tumor growth is unknown. In this study, we found that the subcellular distribution of endogenous ClC-3 varied in a cell cycle-dependent manner in HeLa cells. During interphase, ClC-3 was distributed throughout the cell and it accumulated at various positions in different stages. In early G1, ClC-3 was mainly located in the nucleus. In middle G1, ClC-3 gathered around the nuclear periphery as a ring. In late G1, ClC-3 moved back into the nucleus, where it remained throughout S phase. In G2, ClC-3 was concentrated in the cytoplasm. When cells progressed from G2 to the prophase of mitosis, ClC-3 from the cytoplasm translocated into the nucleus. During metaphase and anaphase, ClC-3 was distributed throughout the cell except for around the chromosomes and was aggregated at the spindle poles and in between two chromosomes, respectively. ClC-3 was then again concentrated in the nucleus upon the progression from telophase to cytokinesis. These results reveal a cell cycle-dependent change of the subcellular distribution of ClC-3 and strongly suggest that ClC-3 has nucleocytoplasmic shuttling dynamics that may play key regulatory roles during different stages of the cell cycle in tumor cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Biol Chem. 2002 Oct 18;277(42):40066-74 - PubMed
    1. Am J Physiol Cell Physiol. 2002 Oct;283(4):C1313-23 - PubMed
    1. Acta Biochim Biophys Sin (Shanghai). 2010 Jun 15;42(6):370-80 - PubMed
    1. J Physiol. 2001 Mar 1;531(Pt 2):437-44 - PubMed
    1. J Am Soc Nephrol. 2009 Jul;20(7):1556-64 - PubMed

Publication types

LinkOut - more resources