Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Jun;37(6):1296-307.
doi: 10.1007/s11064-012-0725-1. Epub 2012 Feb 23.

Plasma membrane-associated glycohydrolases activation by extracellular acidification due to proton exchangers

Massimo Aureli  1 , Nicoletta Loberto, Rosaria Bassi, Anita Ferraretto, Silvia Perego, Patrizia Lanteri, Vanna Chigorno, Sandro Sonnino, Alessandro Prinetti
Affiliations

Plasma membrane-associated glycohydrolases activation by extracellular acidification due to proton exchangers

Massimo Aureli et al. Neurochem Res. 2012 Jun.

Abstract

In this paper, we show that the pH optimum for the plasma membrane (PM)-associated activity of four glycohydrolases (conduritol B epoxide sensitive β-glucosidase, β-glucosidase GBA2, β-hexosaminidase and β-galactosidase) measured on intact cells is acidic. Moreover, we show that drugs able to modify the efflux of protons across the PM, thus locally affecting the extracellular proton concentration close to the PM, are able to modulate the activities of these enzymes. These data strongly suggest that pH-dependent modulation of PM-associated glycohydrolases activities could be an effective way to locally modulate the cell surface glycoconjugate composition.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Expert Rev Neurother. 2009 Oct;9(10):1529-45 - PubMed
    1. Pflugers Arch. 2009 Oct;458(6):1069-83 - PubMed
    1. J Physiol. 2007 Jun 15;581(Pt 3):1235-46 - PubMed
    1. Brain Dev. 2000 May;22(3):196-8 - PubMed
    1. J Neurochem. 1980 Aug;35(2):281-96 - PubMed

Publication types

LinkOut - more resources