Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Aug;64(4):459-64.
doi: 10.1007/s10616-011-9424-z. Epub 2012 Jan 20.

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

Affiliations

Olive leaf extract modulates permethrin induced genetic and oxidative damage in rats

Hasan Turkez et al. Cytotechnology. 2012 Aug.

Abstract

Permethrin is a common synthetic chemical, widely used as an insecticide in agriculture and other domestic applications. The previous reports indicated that permethrin is a highly toxic synthetic pyrethroid pesticide to human and environmental health. Therefore, the present experiment was undertaken to determine the effectiveness of olive leaf extract in modulating the permethrin induced genotoxic and oxidative damage in rats. The animals used were broadly divided into four (A, B, C and D) experimental groups. Group A rats served as control animals and received distilled water intraperitoneally (n = 5). Groups B and C rats received intraperitoneal injections of permethrin (60 mg kg(-1) b.w) and olive leaf extract (500 mg kg(-1) b.w), respectively. Group D rats received permethrin (60 mg kg(-1) b.w) plus olive leaf extract (500 mg kg(-1) b.w). Rats were orally administered their respective feed daily for 21 days. At the end of the experiment rats were anesthetized and serum and bone marrow cell samples were obtained. Genotoxic damage was assessed by micronucleus and chromosomal aberration assays. Total antioxidant capacity and total oxidant status were also measured in serum samples to assess oxidative status. Treatment of Group B with permethrin resulted in genotoxic damage and increased total oxidant status levels. Permethrin treatment also significantly decreased (P < 0.05) total antioxidant capacity level when compared to Group A rats. Group C rats showed significant increases (P < 0.05) in total antioxidant capacity level and no alterations in cytogenetic parameters. Moreover, simultaneous treatments with olive leaf extract significantly modulated the toxic effects of permethrin in Group D rats. It can be concluded that olive leaf extract has beneficial influences and could be able to antagonize permethrin toxicity. As a result, this investigation clearly revealed the protective role of olive leaf extract against the genetic and oxidative damage by permethrin in vivo for the first time.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Assayed ME, Khalaf AA, Salem HA. Protective effects of garlic extract and vitamin C against in vivo cypermethrin-induced cytogenetic damage in rat bone-marrow. Mutat Res. 2010;702:1–7. doi: 10.1016/j.mrgentox.2010.02.020. - DOI - PubMed
    1. Bao J, Zhang DW, Zhang JZ, Huang PL, Huang PL, Lee-Huang S. Computational study of bindings of olive leaf extract (OLE) to HIV-1 fusion protein gp41. FEBS Lett. 2007;12:2737–2742. doi: 10.1016/j.febslet.2007.05.029. - DOI - PubMed
    1. Başer S, Erkoç F, Selvi M, Koçak O. Investigation of acute toxicity of permethrin on guppies Poecilia reticulata. Chemosphere. 2003;51:469–474. doi: 10.1016/S0045-6535(03)00033-X. - DOI - PubMed
    1. Benavente-García O, Castillo J, Lorente J, Alcaraz M. Radioprotective effects in vivo of phenolics extracted from Olea europaea L. leaves against X-ray-induced chromosomal damage: comparative study versus several flavonoids and sulfur-containing compounds. J Med Food. 2002;5:125–135. doi: 10.1089/10966200260398152. - DOI - PubMed
    1. Bouallagui Z, Han J, Isoda H, Sayadi S. Hydroxytyrosol rich extract from olive leaves modulates cell cycle progression in MCF-7 human breast cancer cells. Food Chem Toxicol. 2011;49:179–184. doi: 10.1016/j.fct.2010.10.014. - DOI - PubMed