Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium
- PMID: 2208278
- DOI: 10.1016/0092-8674(90)90300-4
Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium
Abstract
We have identified a novel growth factor, stem cell factor (SCF), for primitive hematopoietic progenitors based on its activity on bone marrow cells derived from mice treated with 5-fluorouracil. The protein was isolated from the medium conditioned by Buffalo rat liver cells. It is heavily glycosylated, with both N-linked and O-linked carbohydrate. Amino acid sequence following removal of N-terminal pyroglutamate is presented. The protein has potent synergistic activities in semisolid bone marrow cultures in conjunction with colony-stimulating factors. It is also a growth factor for mast cells. In two companion papers, we present the sequences of partial SCF cDNAs, identify SCF as a c-kit ligand, and map the SCF gene to the Sl locus of the mouse.
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