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. 2011 May;4(3):299-303.
doi: 10.4161/cib.4.3.14778.

Inhibition of unwinding and ATPase activities of Plasmodium falciparum Dbp5/DDX19 homolog

Jatin Mehta  1 , Renu Tuteja
Affiliations

Inhibition of unwinding and ATPase activities of Plasmodium falciparum Dbp5/DDX19 homolog

Jatin Mehta et al. Commun Integr Biol. 2011 May.

Abstract

We have recently reported the isolation and characterization of Plasmodium falciparum Dbp5/DDX19 homolog PfD66 and the results indicate that it contains ATP-dependent bipolar DNA and RNA unwinding activity, intrinsic nucleic acid-dependent ATPase and RNA-binding activities. In the present study we report the effect of a number of compounds such as actinomycin D, aphidicolin, camptothecin, cyclophosphamide, 4',6'-di-amidino-2-phenylindole (DAPI), daunorubicin, distamycin, ethidium bromide, ellipticine, genistein, mitoxantrone, nalidixic acid, netropsin, nogalamycin, novobiocin and VP-16 on the DNA unwinding and ATPase activities of PfD66. The results indicate that DAPI, ethidium bromide, netropsin and nogalamycin efficiently inhibited the helicase and ATPase activities of PfD66. These studies will make an important contribution in understanding the mechanism of DNA unwinding by Plasmodium falciparum helicase PfD66.

Keywords: ATPase; DNA-binding agents; Plasmodium falciparum; helicase; malaria parasite.

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Figures

Figure 1
Figure 1
The effect of different DNA intercalating compounds on the DNA unwinding activity of PfD66. The reaction was performed using purified enzyme PfD66 and 50 µM of the compound. The DNA-interacting compounds used in the present study were purchased from Topogene Inc., (Columbus, OH), Sigma Chemical Co., (St. Louis, MO) and BDH (E. Merck, Mumbai, India). All of these compounds were dissolved in dimethylsulfoxide (DMSO). The compound added is mentioned at the top of the autoradiogram and (C) is control, no inhibitor is control having enzyme without inhibitor and (B) is the heat denatured substrate. (B–I) Concentration curves of netropsin (B), ethidium bromide (D), DAPI (F) and nogalamycin (H). The DNA helicase reactions were performed by using the same substrate and enzyme as in (A) in the presence of increasing concentration of various compounds. In each (C) is control without enzyme and (B) is the boiled substrate and lanes 1–6 are reactions in the presence of increasing amounts of various compounds. (C, E, G and I) show the quantitative data.
Figure 2
Figure 2
The effect of different DNA intercalating compounds on the ATPase activity of PfD66. The reaction was performed using purified enzyme and 50 µM of the compound. The compound added is mentioned at the top of the autoradiogram and (C) is no enzyme control and lane 1 is control having enzyme without inhibitor. (B–I) Concentration curves of netropsin (B), ethidium bromide (D), DAPI (F) and nogalamycin (H). The assays were performed by using the same substrate and enzyme as in (A) in the presence of increasing concentration of various compounds. In each (C) is control without enzyme and lanes 1–6 are reactions in the presence of increasing amounts of various compounds. (C, E, G and I) show the quantitative data.
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References

    1. Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI. The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature. 2005;434:214–217. - PMC - PubMed
    1. Tuteja R. Malaria-An overview. FEBS J. 2007;274:4670–4679. - PubMed
    1. Todryk SM, Hill AV. Malaria vaccines: the stage we are at. Nat Rev Microbiol. 2007;5:487–489. - PubMed
    1. White NJ. Preventing antimalarial drug resistance through combinations. Drug Resist Updat. 1998;1:3–9. - PubMed
    1. Trape JF. The public health impact of chloroquine resistance in Africa. Am J Trop Med Hyg. 2001;64:12–17. - PubMed

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