Review
doi: 10.1177/0300985811421324.
Epub 2011 Oct 4.
The gene or not the gene--that is the question: understanding the genetically engineered mouse phenotype
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- PMID: 21971987
- DOI: 10.1177/0300985811421324
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Review
The gene or not the gene--that is the question: understanding the genetically engineered mouse phenotype
Vet Pathol.
2012 Jan.
Abstract
Embryonic stem cells have had a significant impact on understanding gene function and gene interactions through the use of genetically engineered mice. However, the genetic context (ie, mouse strain) in which these modifications in alleles are made may have a considerable effect on the phenotypic changes identified in these mice. In addition, tissue- and time-specific gene expression systems may generate unanticipated outcomes. This article discusses the history of embryonic stem cells, reviews how mouse strain can affect phenotype (using specific examples), and examines some of the caveats of conditional gene expression systems.
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