Revision of the oligosaccharide structures of yeast carboxypeptidase Y
- PMID: 2185468
- PMCID: PMC53901
- DOI: 10.1073/pnas.87.9.3368
Revision of the oligosaccharide structures of yeast carboxypeptidase Y
Abstract
The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiae mnn9 mutant bulk mannoprotein. The results support the view that the mnn mutants make oligosaccharides that are a true reflection of the normal biosynthetic pathway and confirm that a recently revised yeast oligosaccharide structure is applicable to wild-type mannoproteins.
Similar articles
-
Localization of alpha 1----3-linked mannoses in the N-linked oligosaccharides of Saccharomyces cerevisiae mnn mutants.Biochemistry. 1990 Mar 13;29(10):2471-82. doi: 10.1021/bi00462a006. Biochemistry. 1990. PMID: 2185827
-
Carbohydrate structure of Saccharomyces cerevisiae mnn9 mannoprotein.J Biol Chem. 1984 Mar 25;259(6):3805-11. J Biol Chem. 1984. PMID: 6368551
-
Structure of the phosphorylated N-linked oligosaccharides from the mnn9 and mnn10 mutants of Saccharomyces cerevisiae.J Biol Chem. 1989 Aug 15;264(23):13648-59. J Biol Chem. 1989. PMID: 2668269
-
Preparative purification of tyrosinamide N-linked oligosaccharides.J Chromatogr A. 1996 Jan 12;720(1-2):235-49. doi: 10.1016/0021-9673(95)00458-0. J Chromatogr A. 1996. PMID: 8601193 Review.
-
Glycosidases of the asparagine-linked oligosaccharide processing pathway.Glycobiology. 1994 Apr;4(2):113-25. doi: 10.1093/glycob/4.2.113. Glycobiology. 1994. PMID: 8054711 Review. No abstract available.
Cited by
-
Yeast glycoprotein biosynthesis: MNT1 encodes an alpha-1,2-mannosyltransferase involved in O-glycosylation.Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6846-50. doi: 10.1073/pnas.89.15.6846. Proc Natl Acad Sci U S A. 1992. PMID: 1495972 Free PMC article.
-
Filamentous fungi as production organisms for glycoproteins of bio-medical interest.Glycoconj J. 1999 Feb;16(2):99-107. doi: 10.1023/a:1026436424881. Glycoconj J. 1999. PMID: 10612410 Review.
-
Localization and targeting of the Saccharomyces cerevisiae Kre2p/Mnt1p alpha 1,2-mannosyltransferase to a medial-Golgi compartment.J Cell Biol. 1995 Nov;131(4):913-27. doi: 10.1083/jcb.131.4.913. J Cell Biol. 1995. PMID: 7490293 Free PMC article.
-
Glycan modification of a thermostable recombinant (1-3, 1-4)-beta-glucanase secreted from Saccharomyces cerevisiae is determined by strain and culture conditions.Glycoconj J. 1995 Jun;12(3):380-90. doi: 10.1007/BF00731341. Glycoconj J. 1995. PMID: 7496153
-
Disruption of the processing alpha-mannosidase gene does not prevent outer chain synthesis in Saccharomyces cerevisiae.Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):21-6. doi: 10.1042/bj2900021. Biochem J. 1993. PMID: 8439291 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases