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Review
. 1990 Mar;54(1):18-51.
doi: 10.1128/mr.54.1.18-51.1990.

Nucleotide excision repair in Escherichia coli

B Van Houten  1
Affiliations
Review

Nucleotide excision repair in Escherichia coli

B Van Houten. Microbiol Rev. 1990 Mar.

Abstract

One of the best-studied DNA repair pathways is nucleotide excision repair, a process consisting of DNA damage recognition, incision, excision, repair resynthesis, and DNA ligation. Escherichia coli has served as a model organism for the study of this process. Recently, many of the proteins that mediate E. coli nucleotide excision have been purified to homogeneity; this had led to a molecular description of this repair pathway. One of the key repair enzymes of this pathway is the UvrABC nuclease complex. The individual subunits of this enzyme cooperate in a complex series of partial reactions to bind to and incise the DNA near a damaged nucleotide. The UvrABC complex displays a remarkable substrate diversity. Defining the structural features of DNA lesions that provide the specificity for damage recognition by the UvrABC complex is of great importance, since it represents a unique form of protein-DNA interaction. Using a number of in vitro assays, researchers have been able to elucidate the action mechanism of the UvrABC nuclease complex. Current research is devoted to understanding how these complex events are mediated within the living cell.

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References

    1. Proc Natl Acad Sci U S A. 1964 Feb;51:226-31 - PubMed
    1. Nucleic Acids Res. 1989 Jun 26;17(12):4689-98 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2558-62 - PubMed
    1. J Biol Chem. 1986 Oct 25;261(30):14135-41 - PubMed
    1. J Mol Biol. 1984 Sep 25;178(3):595-609 - PubMed

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