Interaction of Epstein-Barr viral (EBV) origin of replication (oriP) with EBNA-1 and cellular anti-EBNA-1 proteins
- PMID: 2167556
- DOI: 10.1016/0042-6822(90)90407-i
Interaction of Epstein-Barr viral (EBV) origin of replication (oriP) with EBNA-1 and cellular anti-EBNA-1 proteins
Abstract
We have previously shown that 12-O-tetradecanoylphorbol-13-acetate (TPA) which activates expression of the latent genome of the Epstein-Barr virus (EBV) in Burkitt lymphoma cells induces the synthesis of two cellular anti-EBNA-1 competitor proteins, anti-EBNA-1.1 and anti-EBNA-1.2. Both anti-EBNA-1 proteins can uncouple the specific binding of the EBNA-1 to the region required for EBV plasmid maintenance (oriP). Here, we show by DNase I footprinting that the binding sites on oriP for the EBNA-1 and the anti-EBNA-1 proteins were indistinguishable. The proteins bound to the 30-bp tandem repeats of the oriP. Glycerol-gradient centrifugation and gel retardation assay revealed that a 60-kDa protein formed the anti-EBNA-1.1-DNA complex and a 40-kDa protein formed the anti-EBNA-1.2-DNA complex.
Similar articles
-
Interaction of Epstein-Barr virus nuclear antigen 1 with the viral latent origin of replication.J Virol. 1992 Feb;66(2):694-705. doi: 10.1128/JVI.66.2.694-705.1992. J Virol. 1992. PMID: 1309908 Free PMC article.
-
Interaction of the lymphocyte-derived Epstein-Barr virus nuclear antigen EBNA-1 with its DNA-binding sites.J Virol. 1989 Jan;63(1):101-10. doi: 10.1128/JVI.63.1.101-110.1989. J Virol. 1989. PMID: 2535719 Free PMC article.
-
Role of EBNA-1 in arresting replication forks at the Epstein-Barr virus oriP family of tandem repeats.Mol Cell Biol. 1991 Dec;11(12):6268-78. doi: 10.1128/mcb.11.12.6268-6278.1991. Mol Cell Biol. 1991. PMID: 1658629 Free PMC article.
-
Immortalizing genes of Epstein-Barr virus.Adv Virus Res. 1991;40:19-55. doi: 10.1016/s0065-3527(08)60276-6. Adv Virus Res. 1991. PMID: 1659776 Review.
-
EBNA-1: a protein pivotal to latent infection by Epstein-Barr virus.Rev Med Virol. 2000 Mar-Apr;10(2):83-100. doi: 10.1002/(sici)1099-1654(200003/04)10:2<83::aid-rmv262>3.0.co;2-t. Rev Med Virol. 2000. PMID: 10713596 Review.
Cited by
-
The plasmid replicon of EBV consists of multiple cis-acting elements that facilitate DNA synthesis by the cell and a viral maintenance element.EMBO J. 1998 Nov 2;17(21):6394-403. doi: 10.1093/emboj/17.21.6394. EMBO J. 1998. PMID: 9799247 Free PMC article.
-
The cellular proteins that bind specifically to the Epstein-Barr virus origin of plasmid DNA replication belong to a gene family.Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2843-7. doi: 10.1073/pnas.91.7.2843. Proc Natl Acad Sci U S A. 1994. PMID: 8146198 Free PMC article.
-
Constitutive binding of EBNA1 protein to the Epstein-Barr virus replication origin, oriP, with distortion of DNA structure during latent infection.EMBO J. 1993 Dec 15;12(13):4933-44. doi: 10.1002/j.1460-2075.1993.tb06187.x. EMBO J. 1993. PMID: 8262037 Free PMC article.