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. 2010 Jul;33(3):558-63.
doi: 10.1590/S1415-47572010005000070. Epub 2010 Sep 1.

Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster

Wender Ferreira Costa  1 , Alaide Braga de Oliveira, Júlio César Nepomuceno
Affiliations

Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster

Wender Ferreira Costa et al. Genet Mol Biol. 2010 Jul.

Abstract

This study investigated the genotoxicity of Lapachol (LAP) evaluated by wing spot test of Drosophila melanogaster in the descendants from standard (ST) and high bioactivation (HB) crosses. This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the fly's wings. Drosophila has extensive genetic homology to mammals, which makes it a suitable model organism for genotoxic investigations. Three-day-old larvae from ST crosses (females flr(3)/TM3, Bd(s) x males mwh/mwh), with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross) (females ORR; flr(3)/TM3, Bd(s) x males mwh/mwh), were used. The results showed that LAP is a promutagen, exhibiting genotoxic activity in larvae from the HB cross. In other words, an increase in the frequency of spots is exclusive of individuals with a high level of the cytochrome P450. The results also indicate that recombinogenicity is the main genotoxic event induced by LAP.

Keywords: Drosophila melanogaster; SMART; cytochrome P450; doxorubicin; lapachol.

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Figures

Figure 1
Figure 1
Chemical structures of LAP and DXR.
See this image and copyright information in PMC

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