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. 1990 Jun;64(6):2932-40.
doi: 10.1128/JVI.64.6.2932-2940.1990.

Cytomegalovirus activates transcription directed by the long terminal repeat of human immunodeficiency virus type 1

P A Barry  1 , E Pratt-Lowe, B M Peterlin, P A Luciw
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Cytomegalovirus activates transcription directed by the long terminal repeat of human immunodeficiency virus type 1

P A Barry et al. J Virol. 1990 Jun.

Abstract

Proteins encoded by a variety of DNA viruses activate gene expression from the promoter within the long terminal repeat (LTR) of the human immunodeficiency virus type 1 (HIV-1). The mechanism by which immediate-early (IE) gene products of human cytomegalovirus (CMV) activate expression from the HIV-1 LTR was examined in transient expression assays in cultures of human cells by using plasmids containing the LTR linked to the bacterial chloramphenicol acetyltransferase (CAT) gene and a plasmid expressing the CMV IE gene. Analysis of clustered site mutations within the HIV-1 LTR revealed that sequences from nucleotides -6 to +20 (relative to the start site of transcription) are critical for responsiveness to transactivation by CMV IE gene products. This region partially overlaps the trans-acting response element (+19 to +42) required for function of the HIV-1 transactivator. The CMV IE gene was shown to increase the steady-state levels of both prematurely terminated and full-length transcripts initiated within the LTR. These results support a model in which CMV IE gene products act through a specific regulatory element in the HIV-1 LTR to increase viral transcription.

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