doi: 10.1007/978-1-61779-046-1_7.
Native gel analysis for RISC assembly
Affiliations
- PMID: 21528449
- DOI: 10.1007/978-1-61779-046-1_7
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Native gel analysis for RISC assembly
Methods Mol Biol.
2011.
Abstract
Small-interfering RNAs (siRNAs) and microRNAs (miRNAs) regulate expression of their target mRNAs via the RNA-induced silencing complex (RISC). A core component of RISC is the Argonaute (Ago) protein, which dictates the RISC function. In Drosophila, miRNAs and siRNAs are generally loaded into Ago1-containing RISC (Ago1-RISC) and Ago2-containing RISC (Ago2-RISC), respectively. We developed a native agarose gel system to directly detect Ago1-RISC, Ago2-RISC, and their precursor complexes. Methods presented here will provide powerful tools to biochemically dissect the RISC assembly pathways.
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