Isolation and characterization of plant protein complexes by mass spectrometry
- PMID: 21472857
- DOI: 10.1002/pmic.201000635
Isolation and characterization of plant protein complexes by mass spectrometry
Abstract
The components that enable cells and organisms to fulfill a plethora of chemical and physical reactions, including their ability to metabolize, replicate, repair and communicate with their environment are mostly based on the functioning of highly complex cellular machines which are to a large extent composed of proteins. With the development of MS techniques compatible with the analysis of minute amounts of biological material, it has become more and more feasible to dissect the composition and modification of these protein machineries. Indeed, new purification methods of protein complexes followed by MS analysis together with the genomic sequencing of various organisms - and in particular of crop species - now provide unforeseen insight to understand biological processes at a molecular level. We here review the current state of the art of in vivo protein complex isolation and their MS-based analytical characterization, emphasizing on the tandem affinity purification approach.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Similar articles
-
Linking the proteins--elucidation of proteome-scale networks using mass spectrometry.Mass Spectrom Rev. 2011 Mar-Apr;30(2):268-97. doi: 10.1002/mas.20278. Epub 2010 May 24. Mass Spectrom Rev. 2011. PMID: 21337599 Review.
-
Toward a definition of the complete proteome of plant peroxisomes: Where experimental proteomics must be complemented by bioinformatics.Proteomics. 2011 May;11(9):1764-79. doi: 10.1002/pmic.201000681. Epub 2011 Apr 7. Proteomics. 2011. PMID: 21472859 Review.
-
An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells.Nat Methods. 2006 Dec;3(12):1013-9. doi: 10.1038/nmeth968. Epub 2006 Oct 22. Nat Methods. 2006. PMID: 17060908
-
Recent progress in liquid chromatography-based separation and label-free quantitative plant proteomics.Phytochemistry. 2011 Jul;72(10):963-74. doi: 10.1016/j.phytochem.2010.11.009. Epub 2010 Dec 20. Phytochemistry. 2011. PMID: 21176926 Review.
-
Analysis of plasma membrane proteome in soybean and application to flooding stress response.J Proteome Res. 2009 Oct;8(10):4487-99. doi: 10.1021/pr9002883. J Proteome Res. 2009. PMID: 19658398
Cited by
-
Role of AGC kinases in plant growth and stress responses.Cell Mol Life Sci. 2012 Oct;69(19):3259-67. doi: 10.1007/s00018-012-1093-3. Epub 2012 Jul 31. Cell Mol Life Sci. 2012. PMID: 22847330 Free PMC article. Review.
-
Advances in plant proteomics toward improvement of crop productivity and stress resistancex.Front Plant Sci. 2015 Apr 14;6:209. doi: 10.3389/fpls.2015.00209. eCollection 2015. Front Plant Sci. 2015. PMID: 25926838 Free PMC article. Review.
-
PTEN modulates miR-21 processing via RNA-regulatory protein RNH1.PLoS One. 2011;6(12):e28308. doi: 10.1371/journal.pone.0028308. Epub 2011 Dec 5. PLoS One. 2011. PMID: 22162762 Free PMC article.
-
Elements of the cellular metabolic structure.Front Mol Biosci. 2015 Apr 28;2:16. doi: 10.3389/fmolb.2015.00016. eCollection 2015. Front Mol Biosci. 2015. PMID: 25988183 Free PMC article.
-
Proteomic evaluation of kimchi, a traditional Korean fermented vegetable, and comparison of kimchi manufactured in China and Korea.J Food Sci Technol. 2021 Jan;58(1):389-396. doi: 10.1007/s13197-020-04777-9. Epub 2020 Sep 17. J Food Sci Technol. 2021. PMID: 33505084 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
