Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Apr 6;52(5):2165-9.
doi: 10.1167/iovs.11-7328. Print 2011 Apr.

Age-related macular degeneration and the other double helix. The Cogan Lecture

Jayakrishna Ambati  1
Affiliations

Age-related macular degeneration and the other double helix. The Cogan Lecture

Jayakrishna Ambati. Invest Ophthalmol Vis Sci. .

Abstract

The study and therapy of age-related macular degeneration (AMD), a leading cause of blindness worldwide, have taken great strides over the past decade. During the same time, a central role for RNA in many human diseases has been discovered. We have identified anti-angiogenic functions for synthetic double stranded RNAs (dsRNAs) in neovascular AMD and cytotoxic functions for endogenous dsRNAs in atrophic AMD. These findings provide new insights into the pathogenesis and therapy of both forms of AMD.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Calculating CNV using area measurements may lead to artifactual differences, as determined by precise focal plane during imaging. CNV lesion size is most accurately measured using volumetrics whereby areas derived from a z-stack of focal plane images are summed from the most anterior to posterior plane of the lesion, as seen in the orthogonal section of an FITC-lectin stained choroid 7 days after laser injury (a). When CNV lesion size is calculated by using area-based methodology, there may be large artifactual differences due to the observer choice of focal plane from which the image slice is taken. Thus, depending on whether the operator chosen image slice is selected from the anterior (yellow line), middle (blue line), or posterior (red line) aspect of the lesion (b), there will be large variation in the final area measurement (c–e).
Figure 2.
Figure 2.
Intravitreous Luc siRNA administration leads to loss of GFP signal due to retinal degeneration[b]. Transgenic eGFP mice were treated with four intravitreous 21-nt Luc siRNA injections (1 μg). Dramatic retinal cell death and substantially decreased GFP signal were observed with Luc siRNA-treated eyes (b) compared with the PBS controls (a), as seen on RPE/choroid flat mount preparations. Scale bar, 100 μm.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Kawasaki R, Yasuda M, Song SJ, et al. The prevalence of age-related macular degeneration in Asians: a systematic review and meta-analysis. Ophthalmology. 2010;117:921–927 - PubMed
    1. Krishnan T, Ravindran RD, Murthy GV, et al. Prevalence of early and late age-related macular degeneration in India: the INDEYE study. Invest Ophthalmol Vis Sci. 2010;51:701–707 - PMC - PubMed
    1. Rein DB, Wittenborn JS, Zhang X, Honeycutt AA, Lesesne SB, Saaddine J. Forecasting age-related macular degeneration through the year 2050: the potential impact of new treatments. Arch Ophthalmol. 2009;127:533–540 - PubMed
    1. Smith W, Assink J, Klein R, et al. Risk factors for age-related macular degeneration: pooled findings from three continents. Ophthalmology. 2001;108:697–704 - PubMed
    1. Wilmo A, Prince M. World Alzheimer Report 2010. London: Alzheimer's Disease International; 2010

Personal name as subject