Ontogeny of thymic cortical epithelial cells expressing the thymoproteasome subunit β5t
- PMID: 21469133
- DOI: 10.1002/eji.201041375
Ontogeny of thymic cortical epithelial cells expressing the thymoproteasome subunit β5t
Abstract
Proteasomes are responsible for generating peptides presented by class I MHC molecules of the immune system. β5t, a recently identified proteasome component, is specifically expressed in thymic cortical epithelial cells (cTECs) and plays a pivotal role in generating an immunocompetent repertoire of class I MHC-restricted CD8(+) T cells. Here, we report that β5t is detectable in the thymus as early as E12.5 mouse embryos. We also found that β5t expression in cTECs was detectable in mice deficient for RelB or Rag2, indicating that β5t in cTECs is expressed in the absence of thymic medulla formation or thymocyte development beyond the CD4(-) CD8(-) stage. β5t expression in the embryonic thymus was not detectable in Foxn1-deficient nude mice, although its expression was not reduced in mice deficient for both CCR7 and CCR9, in which fetal thymus colonization by leukocytes is defective. These results indicate that β5t expression in cTECs is dependent on Foxn1 but independent of thymocyte crosstalk or thymic medulla formation.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Similar articles
-
Regulation of CD8+ T cell development by thymus-specific proteasomes.Science. 2007 Jun 1;316(5829):1349-53. doi: 10.1126/science.1141915. Science. 2007. PMID: 17540904
-
Thymoproteasome: probable role in generating positively selecting peptides.Curr Opin Immunol. 2008 Apr;20(2):192-6. doi: 10.1016/j.coi.2008.03.002. Epub 2008 Apr 9. Curr Opin Immunol. 2008. PMID: 18403190 Review.
-
Thymoproteasome shapes immunocompetent repertoire of CD8+ T cells.Immunity. 2010 Jan 29;32(1):29-40. doi: 10.1016/j.immuni.2009.10.009. Epub 2009 Dec 31. Immunity. 2010. PMID: 20045355
-
β5t-containing thymoproteasome: specific expression in thymic cortical epithelial cells and role in positive selection of CD8+ T cells.Curr Opin Immunol. 2012 Feb;24(1):92-8. doi: 10.1016/j.coi.2012.01.006. Epub 2012 Jan 27. Curr Opin Immunol. 2012. PMID: 22285892 Review.
-
Thymoproteasome subunit-β5T generates peptide-MHC complexes specialized for positive selection.Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6979-84. doi: 10.1073/pnas.1222244110. Epub 2013 Apr 8. Proc Natl Acad Sci U S A. 2013. PMID: 23569244 Free PMC article.
Cited by
-
Thymoproteasome optimizes positive selection of CD8+ T cells without contribution of negative selection.Adv Immunol. 2021;149:1-23. doi: 10.1016/bs.ai.2021.03.001. Epub 2021 May 1. Adv Immunol. 2021. PMID: 33993918 Free PMC article. Review.
-
Dynamic spatio-temporal contribution of single β5t+ cortical epithelial precursors to the thymus medulla.Eur J Immunol. 2016 Apr;46(4):846-56. doi: 10.1002/eji.201545995. Epub 2016 Jan 18. Eur J Immunol. 2016. PMID: 26694097 Free PMC article.
-
Progressive Changes in CXCR4 Expression That Define Thymocyte Positive Selection Are Dispensable For Both Innate and Conventional αβT-cell Development.Sci Rep. 2017 Jul 11;7(1):5068. doi: 10.1038/s41598-017-05182-7. Sci Rep. 2017. PMID: 28698642 Free PMC article.
-
Peptides for T cell selection in the thymus.Peptides. 2021 Dec;146:170671. doi: 10.1016/j.peptides.2021.170671. Epub 2021 Oct 5. Peptides. 2021. PMID: 34624431 Free PMC article. Review.
-
Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors.Eur J Immunol. 2013 Mar;43(3):589-94. doi: 10.1002/eji.201243209. Epub 2013 Feb 11. Eur J Immunol. 2013. PMID: 23299414 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials