Enteric adenovirus type 40: expression of E1B mRNA and proteins in permissive and nonpermissive cells
- PMID: 2145689
- DOI: 10.1016/0042-6822(90)90282-v
Enteric adenovirus type 40: expression of E1B mRNA and proteins in permissive and nonpermissive cells
Abstract
The enteric adenovirus type 40 (strain Dugan) grows well in tissue culture only when the E1B 55K protein of Ad5 or Ad12 is supplied in trans, either constitutively expressed in an established cell line or by coinfection with an appropriate helper virus (V. Mautner, N. Mackay, and V. Steinthorsdottir, 1989, Virology 171, 619-622). The synthesis of Ad40 E1B mRNAs and proteins has been examined under permissive and nonpermissive conditions: At late times postinfection in permissive cells, E1B-specific mRNA species of 22 and 13-14 S are made, as well as 15 and 9 S messages for the late IVa2 and ppIX proteins. None of these are detected before the onset of DNA replication and none of them accumulate in the presence of a cytosine arabinoside block to DNA replication. The failure to detect cytoplasmic mRNAs as early times cannot be attributed to a failure of mRNA transport from the nucleus as there is no accumulation of nuclear E1 RNA. In nonpermissive Hela cells only traces of E1B- and ppIX-specific mRNAs are detectable, at very late times postinfection. Antibodies raised to synthetic oligopeptides corresponding to the N- and C-terminal domains of the putative E1B 19K and 55K proteins show a high titer against the cognate peptide by ELISA, but only the E1B 19K C-terminus-specific sera have detected a unique polypeptide in Ad40-infected cells, at late times postinfection. There is no shut-off of host protein synthesis in permissive cells, despite the expression of Ad2 55K protein.
Similar articles
-
Enteric adenovirus type 40:E1B transcription map and identification of novel E1A-E1B cotranscripts in lytically infected cells.Virology. 1991 Mar;181(1):139-49. doi: 10.1016/0042-6822(91)90478-t. Virology. 1991. PMID: 1825250
-
Complementation of enteric adenovirus type 40 for lytic growth in tissue culture by E1B 55K function of adenovirus types 5 and 12.Virology. 1989 Aug;171(2):619-22. doi: 10.1016/0042-6822(89)90634-x. Virology. 1989. PMID: 2527440
-
Cell type specific regulation of expression from the Ad40 E1b promoter in recombinant Ad5/Ad40 viruses.Virology. 1994 Aug 1;202(2):695-706. doi: 10.1006/viro.1994.1391. Virology. 1994. PMID: 8030234
-
The abortive infection of Syrian hamster cells with human adenovirus type 12.Curr Top Microbiol Immunol. 2003;272:415-40. doi: 10.1007/978-3-662-05597-7_14. Curr Top Microbiol Immunol. 2003. PMID: 12747558 Review.
-
The biology of the adenovirus E1B 55K protein.FEBS Lett. 2019 Dec;593(24):3504-3517. doi: 10.1002/1873-3468.13694. Epub 2019 Dec 8. FEBS Lett. 2019. PMID: 31769868 Review.
Cited by
-
A block in release of progeny virus and a high particle-to-infectious unit ratio contribute to poor growth of enteric adenovirus types 40 and 41 in cell culture.J Virol. 1992 May;66(5):3198-205. doi: 10.1128/JVI.66.5.3198-3205.1992. J Virol. 1992. PMID: 1373207 Free PMC article.
-
Subgroup F adenovirus growth in foetal intestinal organ cultures.Arch Virol. 1993;132(1-2):193-200. doi: 10.1007/BF01309853. Arch Virol. 1993. PMID: 8352657
-
Growth of fastidious adenovirus serotype 40 in HRT 18 cells: interactions with E1A and E1B deletion mutants of subgenus C adenoviruses.Arch Virol. 1992;124(1-2):45-56. doi: 10.1007/BF01314624. Arch Virol. 1992. PMID: 1533302 Free PMC article.