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. 2010 Jul 16;1(1):176-185.
doi: 10.1364/BOE.1.000176.

Quantitative comparison of the OCT imaging depth at 1300 nm and 1600 nm

V M Kodach, J Kalkman, D J Faber, T G van Leeuwen

Quantitative comparison of the OCT imaging depth at 1300 nm and 1600 nm

V M Kodach et al. Biomed Opt Express. .

Abstract

One of the present challenges in optical coherence tomography (OCT) is the visualization of deeper structural morphology in biological tissues. Owing to a reduced scattering, a larger imaging depth can be achieved by using longer wavelengths. In this work, we analyze the OCT imaging depth at wavelengths around 1300 nm and 1600 nm by comparing the scattering coefficient and OCT imaging depth for a range of Intralipid concentrations at constant water content. We observe an enhanced OCT imaging depth for 1600 nm compared to 1300 nm for Intralipid concentrations larger than 4 vol.%. For higher Intralipid concentrations, the imaging depth enhancement reaches 30%. The ratio of scattering coefficients at the two wavelengths is constant over a large range of scattering coefficients and corresponds to a scattering power of 2.8 ± 0.1. Based on our results we expect for biological tissues an increase of the OCT imaging depth at 1600 nm compared to 1300 nm for samples with high scattering power and low water content.

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Figures

Fig. 1
Fig. 1
(a) Overview of the time domain OCT set-up used in the experiments: BS – beamsplitter; C1,C2 - fiber collimating ports; L1, L2 - reference and sample arm lenses; M - reference mirror; SMF - single mode fibers; F - long pass filter; PD - photodetector; Lock-in – Lock-in amplifier; PC - personal computer; Fianium – supercontinuum light source; (b) OCT input spectra for the two wavelength bands; (c) coherence function at the two OCT wavelengths (measured with an OD3 filter in sample arm).
Fig. 2
Fig. 2
OCT signals vs. depth for 0.7%, 8.5% and 22.7 vol.% Intralipid samples for the two wavelengths (data before background subtraction and PSF correction).
Fig. 3
Fig. 3
Measured OCT attenuation (a) and scattering (b) coefficients versus Intralipid concentration. The solid lines are visual guides. Error bars depict standard deviations of the measurements.
Fig. 4
Fig. 4
OCT imaging depth for varying Intralipid concentration measured at 1300 and 1600 nm. The solid lines are visual guides. Error bars depict standard deviations of the measurements. Inset: ratio of measured OCT imaging depths. The dashed line indicates equal imaging depth at 1300 and 1600 nm.
Fig. 5
Fig. 5
Measured µs at 1600 nm versus 1300 nm. Sample points are marked according to the Intralipid concentration. From a linear fit to the data (solid line) we determine the SP value for Intralipid (indicated). The dashed lines indicate the 95% confidence interval of the fit.
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