CUG as a mutant start codon for cat-86 and xylE in Bacillus subtilis
- PMID: 2121617
- DOI: 10.1016/0378-1119(90)90478-a
CUG as a mutant start codon for cat-86 and xylE in Bacillus subtilis
Abstract
The cat-86 gene specifies chloramphenicol acetyltransferase (CAT). The cat-86 start codon is UUG, although related genes have AUG as the start codon. Changing the start codon to AUG increased expression of cat-86 by 36% in Bacillus subtilis. Changing the start codon to GUG and CUG decreased expression to 65% and 30%, respectively, of the level obtained when AUG was the start codon. CUG has not been previously shown to function as a start codon in B. subtilis. N-terminal sequencing of purified CAT protein specified by the CUG mutant, revealed that CUG was indeed the start codon and specified methionine. The gene xylE, which specifies catechol 2,3-dioxygenase, has AUG as its start codon. Changing the start codon for xylE to CUG decreased expression by 98%. However, when the ribosome-binding site sequence for xylE was optimized and the spacing between it and the start codon was increased to 8 nucleotides, xylE activity increased to 13% of the activity observed for AUG. CUG did not function efficiently as a start codon for cat-86 in Escherichia coli. These data suggest conditions under which CUG can function, with modest efficiency, as a start codon in B. subtilis.
Similar articles
-
Bacillus subtilis mutant allele sup-3 causes lysine insertion at ochre codons: use of sup-3 in studies of translational attenuation.J Bacteriol. 1989 Oct;171(10):5322-4. doi: 10.1128/jb.171.10.5322-5324.1989. J Bacteriol. 1989. PMID: 2507520 Free PMC article.
-
Constitutive expression of cat-86 associated with a change in the transcription start point.Gene. 1991 Aug 30;105(1):113-7. doi: 10.1016/0378-1119(91)90521-c. Gene. 1991. PMID: 1937000
-
Site in the cat-86 regulatory leader that permits amicetin to induce expression of the gene.J Bacteriol. 1988 Jul;170(7):2933-8. doi: 10.1128/jb.170.7.2933-2938.1988. J Bacteriol. 1988. PMID: 3133355 Free PMC article.
-
Why is start codon selection so precise in eukaryotes?Translation (Austin). 2014 Mar 12;2(1):e28387. doi: 10.4161/trla.28387. eCollection 2014. Translation (Austin). 2014. PMID: 26779403 Free PMC article. Review.
-
Codon usage and lateral gene transfer in Bacillus subtilis.Curr Opin Microbiol. 1999 Oct;2(5):524-8. doi: 10.1016/s1369-5274(99)00011-9. Curr Opin Microbiol. 1999. PMID: 10508724 Review.
Cited by
-
Genetics of streptomycin production in Streptomyces griseus: molecular structure and putative function of genes strELMB2N.Mol Gen Genet. 1991 Dec;231(1):113-23. doi: 10.1007/BF00293829. Mol Gen Genet. 1991. PMID: 1661369
-
A postgenomic appraisal of osmotolerance in Listeria monocytogenes.Appl Environ Microbiol. 2003 Jan;69(1):1-9. doi: 10.1128/AEM.69.1.1-9.2003. Appl Environ Microbiol. 2003. PMID: 12513970 Free PMC article. Review. No abstract available.
-
A variation of the translation attenuation model can explain the inducible regulation of the pBC16 tetracycline resistance gene in Bacillus subtilis.J Bacteriol. 2006 Jul;188(13):4749-58. doi: 10.1128/JB.01937-05. J Bacteriol. 2006. PMID: 16788184 Free PMC article.
-
Ribosome hopping and translational frameshifting are inadequate alternatives to translational attenuation in cat-86 regulation.J Bacteriol. 1991 Dec;173(24):7881-6. doi: 10.1128/jb.173.24.7881-7886.1991. J Bacteriol. 1991. PMID: 1720771 Free PMC article.
-
Environmental regulation of virulence in group A streptococci: transcription of the gene encoding M protein is stimulated by carbon dioxide.J Bacteriol. 1992 Sep;174(17):5693-701. doi: 10.1128/jb.174.17.5693-5701.1992. J Bacteriol. 1992. PMID: 1512202 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous
