Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2011 Mar;278(5):704-15.
doi: 10.1111/j.1742-4658.2011.08005.x. Epub 2011 Jan 28.

Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination

Michael Bieri  1 , Ann H Kwan, Mehdi Mobli, Glenn F King, Joel P Mackay, Paul R Gooley
Affiliations
Free article
Review

Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination

Michael Bieri et al. FEBS J. 2011 Mar.
Free article

Abstract

A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this review, which is intended for non-spectroscopist, we describe major uses of NMR in protein science beyond solution structure determination. After first touching on how NMR can be used to quickly determine whether a mutation induces structural perturbations in a protein, we describe the unparalleled ability of NMR to monitor binding interactions over a wide range of affinities, molecular masses and solution conditions. We discuss the use of NMR to measure the dynamics of proteins at the atomic level and over a wide range of timescales. Finally, we outline new and expanding areas such as macromolecular structure determination in multicomponent systems, as well as in the solid state and in vivo.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

LinkOut - more resources