Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jun;10(6):3224-31.
doi: 10.1128/mcb.10.6.3224-3231.1990.

Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line

S Kim  1 , E H Humphries, L Tjoelker, L Carlson, C B Thompson
Affiliations

Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line

S Kim et al. Mol Cell Biol. 1990 Jun.

Abstract

The chicken immunoglobulin light-chain gene (IgL) encodes only a single variable gene segment capable of recombination. To generate an immune repertoire, chickens diversify this unique rearranged VL gene segment during B-cell development in the bursa of Fabricius. Sequence analysis of IgL cDNAs suggests that both gene conversion events derived from VL segment pseudogene templates (psi VL) and non-template-derived single-base-pair substitutions contribute to this diversity. To facilitate the study of postrecombinational mechanisms of immunoglobulin gene diversification, avian B-cell lines were examined for the ability to diversify their rearranged IgL gene during in vitro passage. One line that retains this ability, the avian leukosis virus-induced bursal lymphoma cell line DT40, has been identified. After passage for 1 year in culture, 39 of 51 randomly sequenced rearranged V-J segments from a DT40 population defined novel subclones of the parental tumor. All cloned V-J segments displayed the same V-J joint, confirming that the observed diversity arose after V-J rearrangement. Most sequence variations that we observed (203 of 220 base pairs) appeared to result from psi VL-derived gene conversion events; 16 of the 17 novel single nucleotide substitutions were transitions. Based on these data, it appears that immunoglobulin diversification during in vitro passage of DT40 cells is representative of the diversification that occurs during normal B-cell development in the bursa of Fabricius.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Cell Biol. 1989 Nov;9(11):4970-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9253-7 - PubMed
    1. J Natl Cancer Inst. 1968 Aug;41(2):373-8 - PubMed
    1. Nature. 1981 Apr 9;290(5806):475-80 - PubMed
    1. Mol Cell Biol. 1982 Apr;2(4):386-400 - PubMed

Publication types

MeSH terms

Substances

Associated data

LinkOut - more resources