Beneficial effects of cannabinoids (CB) in a murine model of allergen-induced airway inflammation: role of CB1/CB2 receptors
- PMID: 21056512
- DOI: 10.1016/j.imbio.2010.09.004
Beneficial effects of cannabinoids (CB) in a murine model of allergen-induced airway inflammation: role of CB1/CB2 receptors
Abstract
The endocannabinoid system (ECS) consists of two cannabinoid (CB) receptors, namely CB(1) and CB(2) receptor, and their endogenous (endocannabinoids) and exogenous (cannabinoids, e.g. delta-9-tetrahydrocannabinol (THC)) ligands which bind to these receptors. Based on studies suggesting a role of THC and the ECS in inflammation, the objective of this study was to examine their involvement in type I hypersensitivity using a murine model of allergic airway inflammation. THC treatment of C57BL/6 wildtype mice dramatically reduced airway inflammation as determined by significantly reduced total cell counts in bronchoalveolar lavage (BAL). These effects were greatest when mice were treated during both, the sensitization and the challenge phase. Furthermore, systemic immune responses were significantly suppressed in mice which received THC during sensitization phase. To investigate a role of CB(1/2) receptors in this setting, we used pharmacological blockade of CB(1) and/or CB(2) receptors by the selective antagonists and moreover CB(1)/CB(2) receptor double-knockout mice (CB(1)(-/-)/CB(2)(-/-)) and found neither significant changes in the cell patterns in BAL nor in immunoglobulin levels as compared to wildtype mice. Our results indicate that the activation of the ECS by applying the agonist THC is involved in the development of type I allergies. However, CB(1)/CB(2) receptor-independent signalling seems likely in the observed results.
Copyright © 2010 Elsevier GmbH. All rights reserved.
Similar articles
-
Targeted deletion of cannabinoid receptors CB1 and CB2 produced enhanced inflammatory responses to influenza A/PR/8/34 in the absence and presence of Delta9-tetrahydrocannabinol.J Leukoc Biol. 2008 Mar;83(3):785-96. doi: 10.1189/jlb.0907618. Epub 2007 Dec 11. J Leukoc Biol. 2008. PMID: 18073275
-
Cannabinoid-mediated elevation of intracellular calcium: a structure-activity relationship.J Pharmacol Exp Ther. 2006 May;317(2):820-9. doi: 10.1124/jpet.105.100503. Epub 2006 Jan 25. J Pharmacol Exp Ther. 2006. PMID: 16436496
-
The inhibitory action of exo- and endocannabinoids on [³H]GABA release are mediated by both CB₁and CB₂receptors in the mouse hippocampus.Neurochem Int. 2012 Jan;60(2):145-52. doi: 10.1016/j.neuint.2011.11.012. Epub 2011 Nov 22. Neurochem Int. 2012. PMID: 22133429
-
Cannabinoids for the treatment of inflammation.Curr Opin Investig Drugs. 2007 May;8(5):373-84. Curr Opin Investig Drugs. 2007. PMID: 17520866 Review.
-
Cannabinoid receptors 1 and 2 (CB1 and CB2), their distribution, ligands and functional involvement in nervous system structures--a short review.Pharmacol Biochem Behav. 2008 Oct;90(4):501-11. doi: 10.1016/j.pbb.2008.05.010. Pharmacol Biochem Behav. 2008. PMID: 18584858 Review.
Cited by
-
Endocannabinoid receptor CB2R is significantly expressed in aspirin-exacerbated respiratory disease: a pilot study.Int Forum Allergy Rhinol. 2018 Oct;8(10):1184-1189. doi: 10.1002/alr.22163. Epub 2018 Jun 13. Int Forum Allergy Rhinol. 2018. PMID: 29897665 Free PMC article.
-
The Impact of the CB2 Cannabinoid Receptor in Inflammatory Diseases: An Update.Molecules. 2024 Jul 18;29(14):3381. doi: 10.3390/molecules29143381. Molecules. 2024. PMID: 39064959 Free PMC article. Review.
-
Anti-Inflammatory and Antiviral Effects of Cannabinoids in Inhibiting and Preventing SARS-CoV-2 Infection.Int J Mol Sci. 2022 Apr 10;23(8):4170. doi: 10.3390/ijms23084170. Int J Mol Sci. 2022. PMID: 35456990 Free PMC article. Review.
-
Cannabinoid receptor 2 augments eosinophil responsiveness and aggravates allergen-induced pulmonary inflammation in mice.Allergy. 2016 Jul;71(7):944-56. doi: 10.1111/all.12858. Epub 2016 Mar 16. Allergy. 2016. PMID: 26850094 Free PMC article.
-
Is lipid signaling through cannabinoid 2 receptors part of a protective system?Prog Lipid Res. 2011 Apr;50(2):193-211. doi: 10.1016/j.plipres.2011.01.001. Epub 2011 Feb 2. Prog Lipid Res. 2011. PMID: 21295074 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources