Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 May;14(5):727-33.
doi: 10.1007/BF00016505.

The minimum functional length of pre-mRNA introns in monocots and dicots

G J Goodall  1 , W Filipowicz
Affiliations

The minimum functional length of pre-mRNA introns in monocots and dicots

G J Goodall et al. Plant Mol Biol. 1990 May.

Abstract

When exogenous genes are to be expressed in transgenic plants, their RNAs must be correctly processed. To gain information useful for predicting whether foreign introns will be accurately spliced, we have analysed the processing of an artificial gene in maize and Nicotiana plumbaginifolia protoplasts. A synthetic plant intron, devised to contain the elements necessary for pre-mRNA splicing in dicots, was found to be efficiently spliced in a monocot (maize) transient expression system. A series of deletion mutants of the synthetic intron was constructed to assess the minimum functional intron length. In both monocots and dicots this was found to be between 70 and 73 nt. This length requirement is similar to that seen in vertebrates, but significantly greater than that in fungi and insects.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. EMBO J. 1986 May;5(5):835-41 - PubMed
    1. Annu Rev Biochem. 1986;55:1119-50 - PubMed
    1. Cell. 1986 Jan 17;44(1):7-19 - PubMed
    1. Cell. 1985 Aug;42(1):355-67 - PubMed
    1. Methods Enzymol. 1990;181:148-61 - PubMed

LinkOut - more resources