Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Apr;32(2):273-83.
doi: 10.1007/s13277-010-0119-4. Epub 2010 Oct 21.

A critical role of Sp1 transcription factor in regulating the human Ki-67 gene expression

Hui Tian  1 , Guo-Wei Qian, Wang Li, Fei-Fei Chen, Jie-Hui Di, Bao-Fu Zhang, Dong-Sheng Pei, Ping Ma, Jun-Nian Zheng
Affiliations

A critical role of Sp1 transcription factor in regulating the human Ki-67 gene expression

Hui Tian et al. Tumour Biol. 2011 Apr.

Abstract

Ki-67 plays a crucial role in cell proliferation as well as maintenance or regulation of cell division. The mechanism governing the Ki-67 gene expression remains unknown. Thus, we cloned the core promoter of the human Ki-67 gene and further investigated its transcriptional regulation. The putative Sp1 binding sites were confirmed by electrophoretic mobility shift assay together with an anti-Sp1 antibody-mediated supershift assay. Deletion mutagenesis and firefly luciferase reporter gene assay demonstrated the essential contribution of Sp1 on transcriptional activation of the Ki-67 gene. In this study, we first confirm that there are three Sp1 binding sites in the Ki-67 core promoter. Two Sp1 sites (one at position -159 to -145 nt and the other at position -14 to +12 nt) are mainly involved in transcriptional regulation of the Ki-67 gene. Overexpression of Sp1 can enhance the Ki-67 promoter activity. However, down-regulation of Sp1 expression using siRNA-Sp1 and mithramycin effectively inhibits the Ki-67 gene transcription. Our results suggest that Sp1 is essential for basal promoter activity of the human Ki-67 gene. Inhibition of the Ki-67 transcriptional activity through abolishment of Sp1 may provide the useful prospect for gene therapy.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cell. 1983 Mar;32(3):669-80 - PubMed
    1. Gene. 1999 Oct 1;238(2):291-300 - PubMed
    1. BJU Int. 2005 Feb;95(3):416-20 - PubMed
    1. BMC Cancer. 2002 Dec 10;2:35 - PubMed
    1. Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6611-6 - PubMed

Publication types

MeSH terms