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. 1978 Apr 1;171(1):263-6.
doi: 10.1042/bj1710263.

Problems encountered in measuring the activity of phosphatidate phosphohydrolase

R G Sturton, D N Brindley

Problems encountered in measuring the activity of phosphatidate phosphohydrolase

R G Sturton et al. Biochem J. .

Abstract

The measurement of phosphate release from phosphatidate overestimates the microsomal activity of phosphatidate phosphohydrolase from rat liver, since phosphate is also produced via the glycerol phosphate that results from the deacylation of phosphatidate. The determination of phosphate production can be a reliable assay for the soluble phosphatidate phosphohydrolase in rat liver, because the glycerol phosphate formed is not hydrolysed under the conditions used.

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