Integrated viral DNA sequences in Epstein-Barr virus-converted human lymphoma lines
- PMID: 205668
- PMCID: PMC525964
- DOI: 10.1128/JVI.25.3.710-718.1978
Integrated viral DNA sequences in Epstein-Barr virus-converted human lymphoma lines
Abstract
Most human lymphoid cell lines contain multiple copies of circular, nonintegrated Epstein-Barr virus (EBV) DNA molecules as well as viral DNA sequences with properties of integrated DNA. The physical state of the EBV DNA in a human lymphoma line that only contains one virus genome equivalent per cell has now been studied by three different methods, neutral CsCl density gradient centrifugation, actinomycin D-CsCl gradient centrifugation, and Hirt fractionation. This cell line, AW-Ramos, has been obtained by EBV infection in vitro of the apparently EBV-negative Ramos lymphoma line. The results indicate that the EBV DNA in AW-Ramos is present exclusively in a linearly integrated form. Similar data were obtained with two other EBV-converted sublines of Ramos cells.
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