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. 2010 May 6:(39):1938.
doi: 10.3791/1938.

The subventricular zone en-face: wholemount staining and ependymal flow

Zaman Mirzadeh  1 , Fiona Doetsch, Kazunobu Sawamoto, Hynek Wichterle, Arturo Alvarez-Buylla
Affiliations

The subventricular zone en-face: wholemount staining and ependymal flow

Zaman Mirzadeh et al. J Vis Exp. .

Abstract

The walls of the lateral ventricles contain the largest germinal region in the adult mammalian brain. The subventricular zone (SVZ) in these walls is an extensively studied model system for understanding the behavior of neural stem cells and the regulation of adult neurogenesis. Traditionally, these studies have relied on classical sectioning techniques for histological analysis. Here we present an alternative approach, the wholemount technique, which provides a comprehensive, en-face view of this germinal region. Compared to sections, wholemounts preserve the complete cytoarchitecture and cellular relationships within the SVZ. This approach has recently revealed that the adult neural stem cells, or type B1 cells, are part of a mixed neuroepithelium with differentiated ependymal cells lining the lateral ventricles. In addition, this approach has been used to study the planar polarization of ependymal cells and the cerebrospinal fluid flow they generate in the ventricle. With recent evidence that adult neural stem cells are a heterogeneous population that is regionally specified, the wholemount approach will likely be an essential tool for understanding the organization and parcellation of this stem cell niche.

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References

    1. Doetsch F, Alvarez-Buylla A. Network of tangential pathways for neuronal migration in adult mammalian brain. Proc. Natl. Acad. Sci. USA. 1996;93:14895–14900. - PMC - PubMed
    1. Shen Q. Adult SVZ stem cells lie in a vascular niche: a quantitative analysis of niche cell-cell interactions. Cell Stem Cell. 2008;3:289–300. - PMC - PubMed
    1. Tavazoie M. A specialized vascular niche for adult neural stem cells. Cell Stem Cell. 2008;3:279–288. - PMC - PubMed
    1. Mirzadeh Z, Merkle FT, Soriano-Navarro M, Garcia-Verdugo JM, Alvarez-Buylla A. Neural stem cells confer unique pinwheel architecture to the ventricular surface in neurogenic regions of the adult brain. Cell Stem Cell. 2008;3:265–278. - PMC - PubMed
    1. Mirzadeh Z, Han YG, Soriano-Navarro M, Garcia-Verdugo JM, Alvarez-Buylla A. Cilia organize ependymal planar polarity. J Neurosci. 2010;30:2600–2610. - PMC - PubMed

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