Mal/SRF is dispensable for cell proliferation in Drosophila
- PMID: 20404916
- PMCID: PMC2852404
- DOI: 10.1371/journal.pone.0010077
Mal/SRF is dispensable for cell proliferation in Drosophila
Abstract
The Mal/SRF transcription factor is regulated by the level of G-actin in cells and has important roles in cell migration and other actin-dependent processes in Drosophila. A recent report suggests that Mal/SRF and an upstream regulator, Pico, are required for cell proliferation and tissue growth in Drosophila. I find otherwise. Mutation of Mal or SRF does not affect cell proliferation in the fly wing. Furthermore, I cannot reproduce the reported effects of Pico RNAi or Pico overexpression on body size. Nevertheless, I can confirm that overexpression of Pico or Mal causes tissue overgrowth specifically in the fly wing--where SRF is most highly expressed. My results indicate that Mal/SRF can promote tissue growth when abnormally active, but is not normally required for tissue growth during development.
Conflict of interest statement
Figures




Similar articles
-
Drosophila pico and its mammalian ortholog lamellipodin activate serum response factor and promote cell proliferation.Dev Cell. 2008 Nov;15(5):680-90. doi: 10.1016/j.devcel.2008.09.020. Dev Cell. 2008. PMID: 19000833 Free PMC article.
-
Evidence for tension-based regulation of Drosophila MAL and SRF during invasive cell migration.Dev Cell. 2004 Jul;7(1):85-93. doi: 10.1016/j.devcel.2004.05.020. Dev Cell. 2004. PMID: 15239956
-
Transcriptional responses to hyperplastic MRL signalling in Drosophila.Open Biol. 2017 Feb;7(2):160306. doi: 10.1098/rsob.160306. Open Biol. 2017. PMID: 28148822 Free PMC article.
-
Serum response factor: master regulator of the actin cytoskeleton and contractile apparatus.Am J Physiol Cell Physiol. 2007 Jan;292(1):C70-81. doi: 10.1152/ajpcell.00386.2006. Epub 2006 Aug 23. Am J Physiol Cell Physiol. 2007. PMID: 16928770 Review.
-
A nuclear MAL-function links Rho to SRF.Mol Cell. 2003 May;11(5):1121-3. doi: 10.1016/s1097-2765(03)00189-8. Mol Cell. 2003. PMID: 12769835 Review.
Cited by
-
Validating RNAi phenotypes in Drosophila using a synthetic RNAi-resistant transgene.PLoS One. 2013 Aug 8;8(8):e70489. doi: 10.1371/journal.pone.0070489. eCollection 2013. PLoS One. 2013. PMID: 23950943 Free PMC article.
-
Transcriptomic Analysis Suggests Genes Expressed Stage-Independently and Stage-Dependently Modulating the Wing Dimorphism of the Brown Planthopper.Genes (Basel). 2019 Dec 23;11(1):19. doi: 10.3390/genes11010019. Genes (Basel). 2019. PMID: 31878073 Free PMC article.
References
-
- Hafen E. Interplay between growth factor and nutrient signaling: lessons from Drosophila TOR. Curr Top Microbiol Immunol. 2004;279:153–167. - PubMed
-
- Affolter M, Basler K. The Decapentaplegic morphogen gradient: from pattern formation to growth regulation. Nat Rev Genet. 2007;8:663–674. - PubMed
-
- Harvey K, Tapon N. The Salvador-Warts-Hippo pathway - an emerging tumour-suppressor network. Nat Rev Cancer. 2007;7:182–191. - PubMed
-
- Vartiainen MK, Guettler S, Larijani B, Treisman R. Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL. Science. 2007;316:1749–1752. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous