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. 2010 Jun;155(6):983-6.
doi: 10.1007/s00705-010-0665-5. Epub 2010 Apr 9.

Isolation and sequence analysis of Culex flavivirus from Culex interrogator and Culex quinquefasciatus in the Yucatan Peninsula of Mexico

Rungrat Saiyasombat  1 , Karin S Dorman, Julian E Garcia-Rejon, Maria A Loroño-Pino, Jose A Farfan-Ale, Bradley J Blitvich
Affiliations

Isolation and sequence analysis of Culex flavivirus from Culex interrogator and Culex quinquefasciatus in the Yucatan Peninsula of Mexico

Rungrat Saiyasombat et al. Arch Virol. 2010 Jun.

Abstract

Previously, we reported a high prevalence of Culex flavivirus (CxFV) in Culex quinquefasciatus (Say) in the Yucatan Peninsula of Mexico. To determine whether other Culex spp. mosquitoes in this region are susceptible to natural CxFV infection, Cx. bahamensis (Dyar and Knab), Cx. coronator (Dyar and Knab), Cx. interrogator (Dyar and Knab), Cx. nigripalpus (Theobald) and Cx. opisthopus (Komp) in the Yucatan Peninsula of Mexico were tested for CxFV. Two pools of Cx. interrogator were positive. The envelope protein genes of these isolates and 16 isolates from Cx. quinquefasciatus were sequenced and shown to have > or =99.2% nucleotide identity. These data suggest that there is limited genetic diversity among CxFV isolates in the Yucatan Peninsula of Mexico.

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Figures

Fig. 1
Fig. 1
Phylogenetic analysis of the envelope protein gene of 18 CxFV isolates obtained in this study and 16 other CxFV isolates. The envelope protein gene encompasses nucleotide positions 938–2,217 relative to the genomic sequence of the prototype CxFV strain (Tokyo). The displayed phylogeny was estimated by using the program MRBAYES, version 3.1 [23]. Posterior support (out of 100) for selected branches is indicated. An unrooted tree was inferred but is shown rooted using the midpoint method. CxFV isolates obtained in the present study are denoted in bold. GenBank accession numbers for sequences used in the phylogenetic analysis are: M-2168 (GU289684), M-2313 (GU289685), M-2605 (GU289686), M-2614 (GU289687), M-2617 (GU289688), M-2618 (GU289689), M-2630 (GU289690), M-2635 (GU289691), M-2636 (GU289692), M-2637 (GU289693), M-2644 (GU289694), M-2645 (GU289695), M-2648 (GU289696), M-2650 (GU289697), M-2656 (GU289698), M-2663 (GU289699), M-2665 (GU289700), T-955 (GU289683), Mex07 (EU879060.1), Guatemala (EU805805.1), Iowa07 (FJ663034.1), Iowa 1064 (FJ663026.1), Iowa 318 (FJ663030.1), Iowa 599 (FJ663032.1), HOU24284 (FJ502997.1), HOU24519 (FJ502996.1), HOU24516 (FJ502999.1), Hokkaido (AB262762.1), Osaka (AB262763.1), Sura-baya (AB262766.1), Tokyo (AB262759.2), TR3115 (FJ503002.1), TR3116 (FJ503003.1) and Uganda (GQ165808.1)
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